大鼠后肢肌肉在出血性脑卒中后 4 周时的纤维类型适应性的明显差异。
Distinct Patterns of Fiber Type Adaptation in Rat Hindlimb Muscles 4 Weeks After Hemorrhagic Stroke.
机构信息
From the Division of Physical Therapy, Department of Rehabilitation Medicine, University of Minnesota, Minneapolis, Minnesota (LMS); Department of Neurosurgery, University of Minnesota, Minneapolis, Minnesota (WCL); and Department of Physical Therapy and Athletic Training, Boston University, Boston, Massachusetts (LVT).
出版信息
Am J Phys Med Rehabil. 2019 Apr;98(4):266-274. doi: 10.1097/PHM.0000000000001062.
OBJECTIVE
The aim of this study was to evaluate adaptations in soleus and tibialis anterior muscles in a rat model 4 wks after hemorrhagic stroke.
DESIGN
Young adult Sprague Dawley rats were randomly assigned to two groups: stroke and control, with eight soleus and eight tibialis anterior muscles per group. Hemorrhagic stroke was induced in the right caudoputamen of the stroke rats. Control rats had no intervention. Neurologic status was evaluated in both groups before stroke and 4 wks after stroke. Muscles were harvested after poststroke neurologic testing. Muscle fiber types and cross-sectional areas were determined in soleus and tibialis anterior using immunohistochemical labeling for myosin heavy chain.
RESULTS
No generalized fiber atrophy was found in any of the muscles. Fiber types shifted from faster to slower in the tibialis anterior of the stroke group, but no fiber type shifts occurred in the soleus muscles of stroke animals.
CONCLUSIONS
Because slower myosin heavy chain fiber types are associated with weaker contractile force and slower contractile speed, this faster to slower fiber type shift in tibialis anterior muscles may contribute to weaker and slower muscle contraction in this muscle after stroke. This finding may indicate potential therapeutic benefit from treatments known to influence fiber type plasticity.
目的
本研究旨在评估出血性中风后 4 周大鼠模型中比目鱼肌和胫骨前肌的适应性变化。
设计
将年轻成年 Sprague Dawley 大鼠随机分为两组：中风组和对照组，每组各有 8 只比目鱼肌和 8 只胫骨前肌。通过向中风大鼠右侧尾状核内诱导出血性中风。对照组大鼠不进行任何干预。两组大鼠在中风前和中风后 4 周均进行神经功能状态评估。在进行中风后神经功能测试后，采集肌肉。使用肌球蛋白重链的免疫组织化学标记法，确定比目鱼肌和胫骨前肌中的肌纤维类型和横截面积。
结果
未发现任何肌肉发生普遍的纤维萎缩。中风组胫骨前肌中的纤维类型从快肌向慢肌转变，但中风动物的比目鱼肌中未发生纤维类型转变。
结论
由于较慢的肌球蛋白重链纤维类型与较弱的收缩力和较慢的收缩速度相关，因此胫骨前肌中的这种从快肌到慢肌的纤维类型转变可能导致中风后该肌肉收缩力减弱和收缩速度减慢。这一发现可能表明，已知能影响纤维类型可塑性的治疗方法可能具有潜在的治疗益处。
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