van Zoelen E J, van Rooijen M A, van Oostwaard T M, de Laat S W
Cancer Res. 1987 Mar 15;47(6):1582-7.
Cellular transformation of normal rat kidney (NRK) cells by simian sarcoma virus (SSV) results in a complete loss of the cellular requirement of externally added polypeptide growth factors for proliferation. Moreover, SSV-transformed NRK cells have a strongly reduced ability to bind both external platelet-derived growth factor and epidermal growth factor, when compared to nontransformed NRK cells. Analysis of serum-free medium conditioned by SSV-transformed NRK cells shows that this cell line secretes both types alpha and beta transforming growth factor (TGF). The level of TGF alpha production (300 ng/liter conditioned medium) by SSV-transformed NRK is among the highest described to date. Since addition of TGF alpha and beta in combination is sufficient to induce phenotypic transformation of NRK cells, it is concluded that although expression of the sis oncogene is essential for transformation, expression of additional genes may be required for the phenotypic alterations accompanying complete cellular transformation.
猿猴肉瘤病毒（SSV）对正常大鼠肾（NRK）细胞的细胞转化导致细胞对外部添加的多肽生长因子增殖需求的完全丧失。此外，与未转化的NRK细胞相比，SSV转化的NRK细胞结合外部血小板衍生生长因子和表皮生长因子的能力大幅降低。对SSV转化的NRK细胞条件无血清培养基的分析表明，该细胞系分泌α型和β型转化生长因子（TGF）。SSV转化的NRK产生TGFα的水平（300 ng/升条件培养基）是迄今为止报道的最高水平之一。由于联合添加TGFα和β足以诱导NRK细胞的表型转化，因此得出结论，虽然sis癌基因的表达对于转化至关重要，但伴随完全细胞转化的表型改变可能还需要其他基因的表达。
