Carrasco-Ruiz María de Los Ángeles, Hernández-Aragón Laura G, Chávez-Ríos Jesús Ramsés, Rodríguez-Antolín Jorge, Pacheco Pablo, Martínez-Gómez Margarita, Cuevas-Romero Estela, Castelán Francisco
Centro Tlaxcala de Biología de la Conducta, Universidad Autónoma de Tlaxcala, Tlaxcala, México.
Instituto de Ciencias y Fisiología, Benemérita Universidad Autónoma de Puebla, Puebla, México.
Int Neurourol J. 2018 Sep;22(3):161-168. doi: 10.5213/inj.1836116.058. Epub 2018 Sep 28.
To characterize the relationship between serum estradiol levels and the expression of glucose transporter type 4 (Glut4) in the pubococcygeus and iliococcygeus muscles in female rats.
The muscles were excised from virgin rats during the metestrus and proestrus stages of the estrous cycle, and from sham and ovariectomized rats implanted with empty or estradiol benzoate-filled capsules. The expression of estrogen receptors (ERs) was inspected in the muscles at metestrus and proestrus. Relative Glut4 expression, glycogen content, and serum glucose levels were measured. Appropriate statistical tests were done to identify significant differences (P≤0.05).
The pubococcygeus and iliococcygeus muscles expressed ERα and ERβ. Glut4 expression and glycogen content in the pubococcygeus muscle were higher at proestrus than at metestrus. No significant changes were observed in the iliococcygeus muscle. In ovariectomized rats, the administration of estradiol benzoate increased Glut4 expression and glycogen content in the pubococcygeus muscle alone.
High serum estradiol levels increased Glut4 expression and glycogen content in the pubococcygeus muscle, but not in the iliococcygeus muscle.
探讨雌性大鼠血清雌二醇水平与耻骨尾骨肌和髂尾骨肌中葡萄糖转运蛋白4（Glut4）表达之间的关系。
从处于动情周期间情期和动情前期的未交配大鼠，以及假手术和卵巢切除术后分别植入空胶囊或苯甲酸雌二醇填充胶囊的大鼠身上切除肌肉。检测间情期和动情前期肌肉中雌激素受体（ERs）的表达。测量相对Glut4表达、糖原含量和血清葡萄糖水平。进行适当的统计检验以确定显著差异（P≤0.05）。
耻骨尾骨肌和髂尾骨肌表达ERα和ERβ。耻骨尾骨肌中Glut4表达和糖原含量在动情前期高于间情期。髂尾骨肌未观察到显著变化。在卵巢切除的大鼠中，苯甲酸雌二醇的给药仅增加了耻骨尾骨肌中Glut4的表达和糖原含量。
高血清雌二醇水平增加了耻骨尾骨肌中Glut4的表达和糖原含量，但未增加髂尾骨肌中的表达和含量。
