Yeon Min Ji, Lee Min Ho, Kim Do Hyun, Yang Ji Yeong, Woo Hyun Jun, Kwon Hye Jin, Moon Cheol, Kim Sa-Hyun, Kim Jong-Bae
a Department of Biomedical Laboratory Science, College of Health Sciences , Yonsei University , Wonju , Republic of Korea.
b Forensic DNA Division , National Forensic Service , Wonju , Republic of Korea.
Biosci Biotechnol Biochem. 2019 Jan;83(1):166-173. doi: 10.1080/09168451.2018.1528140. Epub 2018 Oct 5.
Inflammation induced by Helicobacter pylori infection related to gastric carcinogenesis. In this study, we have investigated the anti-inflammatory effect and its mechanism of kaempferol in the inflammatory response caused by H. pylori infection in vitro. We found that kaempferol reduced the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and IL-8) and production of IL-8 in AGS cells. In addition, kaempferol suppressed translocation of cytotoxin-associated gene A (CagA) and vacuolating cytotoxin A (VacA) of H. pylori to AGS cells. It was due to decreased transcription of type IV secretion system (T4SS) components involved in CagA injection and secretion system subunit protein A (SecA) of type V secretion system (T5SS) involved in VacA secretion by kaempferol. In conclusion, kaempferol shows the anti-inflammatory effect by suppressing the translocation of CagA and VacA proteins and leading to the down-regulation of pro-inflammatory cytokines. Abbreviations: CagA: cytotoxin-associated gene A; VacA: vacuolating cytotoxin A; T4SS: type IV secretion systems; SecA: secretion system subunit protein A; T5SS: type V secretion system.
幽门螺杆菌感染诱导的炎症与胃癌发生相关。在本研究中，我们调查了山奈酚在体外对幽门螺杆菌感染引起的炎症反应的抗炎作用及其机制。我们发现山奈酚降低了AGS细胞中促炎细胞因子（TNF-α、IL-1β和IL-8）的表达以及IL-8的产生。此外，山奈酚抑制了幽门螺杆菌的细胞毒素相关基因A（CagA）和空泡毒素A（VacA）向AGS细胞的转位。这是由于山奈酚降低了参与CagA注射的IV型分泌系统（T4SS）组件以及参与VacA分泌的V型分泌系统（T5SS）的分泌系统亚基蛋白A（SecA）的转录。总之，山奈酚通过抑制CagA和VacA蛋白的转位并导致促炎细胞因子的下调而显示出抗炎作用。缩写：CagA：细胞毒素相关基因A；VacA：空泡毒素A；T4SS：IV型分泌系统；SecA：分泌系统亚基蛋白A；T5SS：V型分泌系统。
