Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Beijing, 100049, China.
Glycochemistry & Glycobiology Lab, Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 201203, China; University of Chinese Academy of Sciences, No.19(A) Yuquan Road, Beijing, 100049, China.
Carbohydr Polym. 2018 Dec 15;202:134-142. doi: 10.1016/j.carbpol.2018.08.098. Epub 2018 Aug 26.
Previous studies showed that polysaccharides from flowers of Carthamus tinctorius L. had immunomodulating activities. However, their structures were little known. Here, an arabinogalactan, named as HH1-1 with a relative molecular weight of 70.9 kDa, was isolated from flowers of Carthamus tinctorius L. by hot water extraction, followed by purification of a DEAE-Cellulose 32 column and a Sephacryl S-200HR column, subsequently. The structure of HH1-1 was evaluated to have a backbone of 1,6-linked Galp branched at C-3 by side chain of 1,3-linked Galp, with sub-branches attached at its C-3 position. The branches mainly contained 1,5-linked, 1,3,5 linked, terminal arabinose and terminal galactose. Biological activity assays showed that this polysaccharide had immune-enhancing activity by multiple ways, including promoting immune responses by activation of NF-κB signaling and increasing TNF-α, IL-1β and iNOS expressions in lymphocytes and macrophages.
先前的研究表明，红花多糖具有免疫调节活性。然而，它们的结构知之甚少。在这里，从红花中分离得到一种阿拉伯半乳聚糖，命名为 HH1-1，其相对分子量为 70.9kDa。通过热水提取，然后通过 DEAE-Cellulose 32 柱和 Sephacryl S-200HR 柱进行纯化。HH1-1 的结构被评估为具有 1,6-连接的半乳糖作为主链，在 C-3 位置由侧链 1,3-连接的半乳糖分支，在其 C-3 位置有支链。支链主要含有 1,5-连接、1,3,5-连接、末端阿拉伯糖和末端半乳糖。生物活性测定表明，该多糖通过多种途径具有免疫增强活性，包括通过激活 NF-κB 信号通路促进免疫反应，并增加淋巴细胞和巨噬细胞中 TNF-α、IL-1β 和 iNOS 的表达。
