College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, PR China.
Department of Oncology, Qingyang First People's Hospital, Qingyang 745000, PR China.
Carbohydr Polym. 2015;127:94-100. doi: 10.1016/j.carbpol.2015.03.066. Epub 2015 Mar 28.
A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively. The homogeneity and molecular weight were determined using HPGPC. The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy. The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da. The main chain of ABPS-21 was made up of → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 →, and two side chains α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → and α-D-galpA-(1 → were attached to the backbone chain at O-6 position of 1,4,6-linked β-D-Glcp and 1,4,6-linked β-D-Galp, respectively. ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now.
一种水溶性多糖，命名为 ABPS-21，从短梗五加茎皮中通过热水提取分离出来，分别通过纤维素 DEAE-52 和 Sephacryl S-300 HR 凝胶过滤层析进行纯化。通过 HPGPC 确定其均一性和分子量。根据单糖组成和甲基化分析、部分酸水解、高碘酸氧化、Smith 降解、IR 和 NMR 光谱解析，阐明了其结构。结果表明，ABPS-21 是一种均一的杂多糖，由半乳糖、葡萄糖、鼠李糖和半乳糖醛酸以摩尔比 3.0:2.0:2.0:1.0 组成，平均分子量为 1.06×10^5Da。ABPS-21 的主链由 → 4)-β-D-Galp-(1 → 4)-β-D-Glcp-(1 → 4)-β-D-Galp-(1 → 4)-β-D-Galp-(1 → 2)-α-L-Rhap-(1 → 组成，两个侧链α-l-Rhap-(1 → 4)-β-D-Glcp-(1 → 和 α-D-galpA-(1 → 通过 O-6 位与 1,4,6-连接的β-D-Glcp 和 1,4,6-连接的β-D-Galp 连接到主链上。ABPS-21 是一种从短梗五加中分离得到的酸性支链多糖，迄今为止尚未有报道。
