Bates G P, Price R G
Coll Relat Res. 1986 Oct;6(4):325-32. doi: 10.1016/s0174-173x(86)80003-6.
Prolylhydroxylase and galactosylhydroxylsyl glucosyl (GGH) transferase were assayed in cells cultured from cartilage and synovium (adipose type) removed from normal and adjuvant arthritic rat knees. There was a significant increase in prolylhydroxylase in chondrocytes from arthritic knees compared to the normals but no change in GGH transferase activity was found. In the cells derived from the synovium no variation in either enzyme was observed. No significant changes in DNA or protein content was found in either type of cells from the arthritic joints. Comparison of the prolylhydroxylase activity in the supernatant and pellet fractions prepared from chondrocytes from normal and arthritic joints, demonstrated that the majority of the activity was recovered in the supernatant fraction rather than the pellet in arthritic chondrocytes. The data presented indicate that the degree of hydroxylation of cartilage collagen alters in arthritis.
在从正常大鼠和佐剂性关节炎大鼠膝关节取出的软骨和滑膜（脂肪型）培养的细胞中，对脯氨酰羟化酶和半乳糖基羟赖氨酰葡糖（GGH）转移酶进行了测定。与正常大鼠相比，关节炎大鼠膝关节软骨细胞中的脯氨酰羟化酶显著增加，但未发现GGH转移酶活性有变化。在源自滑膜的细胞中，两种酶均未观察到变化。在关节炎关节的两种细胞类型中，DNA或蛋白质含量均未发现显著变化。对正常和关节炎关节软骨细胞制备的上清液和沉淀部分中的脯氨酰羟化酶活性进行比较，结果表明，在关节炎软骨细胞中，大部分活性存在于上清液部分而非沉淀部分。所呈现的数据表明，关节炎中软骨胶原蛋白的羟化程度发生了改变。
