Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Life Sci. 2018 Nov 1;212:203-212. doi: 10.1016/j.lfs.2018.09.052. Epub 2018 Oct 1.
Phagocytic clearance of apoptotic cells (Efferocytosis) could affect the polarization of macrophages and promote M2 anti-inflammatory and regulatory phenotype and function. Here we tested the hypothesis that efferocytosis of apoptotic Adipose-Derived Mesenchymal Stem Cells (AD-MSCs) promotes macrophage M2 polarization. In this study, Macrophages were incubated with apoptotic MSCs and after 48 h interleukin-10 (IL-10), transforming growth factor-alpha (TNFα), and nitric oxide (NO) production were measured. Furthermore, phagocytosis ability and arginase activity were analyzed. The results showed that apoptotic MSCs could reduce TNFα and NO production, and increase IL-10 levels. Moreover, arginase activity and phagocytosis ability were also increased in tested macrophages compared to controls. In Conclusion efferocytosis of AD-MSCs can alter the macrophages phenotype toward regulatory and anti-inflammatory phenotype.
凋亡细胞的吞噬清除（吞噬作用）可能会影响巨噬细胞的极化，并促进 M2 抗炎和调节表型及功能。在这里，我们验证了这样一个假设，即凋亡脂肪间充质干细胞（AD-MSCs）的吞噬作用促进了巨噬细胞 M2 极化。在这项研究中，将巨噬细胞与凋亡的 MSC 共孵育 48 小时后，测量白细胞介素 10（IL-10）、转化生长因子-α（TNFα）和一氧化氮（NO）的产生。此外，还分析了吞噬作用能力和精氨酸酶活性。结果表明，凋亡的 MSC 可以减少 TNFα 和 NO 的产生，并增加 IL-10 的水平。此外，与对照组相比，测试的巨噬细胞中的精氨酸酶活性和吞噬作用能力也有所增加。综上所述，AD-MSCs 的吞噬作用可以改变巨噬细胞的表型，使其向调节和抗炎表型转变。
