Gordon J, Webb A J, Walker L, Guy G R, Rowe M
Eur J Immunol. 1986 Dec;16(12):1627-30. doi: 10.1002/eji.1830161225.
Low molecular weight B cell growth factor (BCGF) and a monoclonal antibody (MHM6) to the 45-kDa, B lineage-restricted, CD23 activation antigen (BLAST-2; EBVCS) were found to be indistinguishable in their biological effects. Individually, both augmented DNA synthesis in activated, but not resting, B lymphocytes while no additional enhancement resulted from using the two agonists in combination. Furthermore, by increasing the expression of Tac, both MHM6 and BCGF promoted activated B cells to respond more vigorously to the late addition of recombinant interleukin 2. The presence of BCGF during B cell activations was found to down-regulate the expression of the CD23 antigen while the coating of activated cells with MHM6 antibody diminished their capacity to absorb BCGF activity. The findings demonstrate that CD23 and a low molecular weight BCGF deliver a comparable growth-promoting signal to activated B cells. A possible relationship between CD23 and the receptor for the low molecular weight BCGF is discussed.
低分子量B细胞生长因子（BCGF）和一种针对45 kDa、B细胞系限制性、CD23激活抗原（BLAST-2；EBVCS）的单克隆抗体（MHM6），在生物学效应上被发现难以区分。单独使用时，二者均可增强活化的而非静止的B淋巴细胞中的DNA合成，而联合使用这两种激动剂并未产生额外增强效果。此外，通过增加Tac的表达，MHM6和BCGF均促使活化的B细胞对后期添加的重组白细胞介素2产生更强烈的反应。在B细胞活化过程中发现BCGF的存在会下调CD23抗原的表达，而用MHM6抗体包被活化细胞会降低其吸收BCGF活性的能力。这些发现表明，CD23和低分子量BCGF向活化的B细胞传递了相当的促生长信号。文中还讨论了CD23与低分子量BCGF受体之间可能存在的关系。
