Mishra G C, Berton M T, Oliver K G, Krammer P H, Uhr J W, Vitetta E S
J Immunol. 1986 Sep 1;137(5):1590-8.
This study describes the generation of a monoclonal mouse x rat antibody (G-48) that recognizes a determinant on the serologically defined LFA-1 alpha-chain. It immunoprecipitates two noncovalently associated polypeptides of 176,000 and 95,000 Mr respectively from lysates of radioiodinated BCL1 cells, T cells, and B cells. G-48 mimics the biological actions of BSF-1 by inducing increased levels of Ia antigen expression on resting B cells, augmenting the proliferation of anti-delta-stimulated B cells, and in insolubilized form, inducing IgG1 secretion by LPS-activated B cells. G-48 does not have BCDF mu, BCGF II, nor IL 2 activity. These results demonstrate that LFA-1 plays an important role in B cell activation, proliferation, and differentiation.
本研究描述了一种单克隆小鼠x大鼠抗体（G-48）的产生，该抗体识别血清学定义的淋巴细胞功能相关抗原-1（LFA-1）α链上的一个决定簇。它从经放射性碘化的BCL1细胞、T细胞和B细胞裂解物中分别免疫沉淀出两条非共价结合的多肽，分子量分别为176,000和95,000道尔顿。G-48通过诱导静止B细胞上Ia抗原表达水平升高、增强抗δ刺激的B细胞增殖以及以不溶性形式诱导脂多糖激活的B细胞分泌IgG1，模拟了B细胞刺激因子-1（BSF-1）的生物学作用。G-48不具有B细胞分化因子μ（BCDFμ）、B细胞生长因子II（BCGF II）或白细胞介素2（IL 2）活性。这些结果表明LFA-1在B细胞活化、增殖和分化中起重要作用。
