Gordon J, Rowe M, Walker L, Guy G
Eur J Immunol. 1986 Sep;16(9):1075-80. doi: 10.1002/eji.1830160908.
CD23,p45 (BLAST-2,EBVCS) is a 45-kDa lineage-restricted antigen which appears on the surface of human B cells shortly after activation. A monoclonal antibody (MHM6) to CD23,p45, as well as a polyclonal rabbit antibody raised against the purified antigen were found to promote DNA synthesis in purified tonsillar B cells which had been activated with phorbol ester. Interleukin 1, which was not, by itself, stimulatory for either resting or activated B cells, significantly augmented the growth-promoting properties of MHM6. Kinetic studies indicated that while MHM6 exerted its influence in early G1, interleukin 1 acted later in the cycle just prior to the entry of cells into S phase. The findings demonstrate a role for CD23,p45 in triggering the progression of activated B lymphocytes through the G1 phase of the cell cycle. The possibility that this antigen serves as a receptor for a B cell stimulatory factor is discussed.
CD23，p45（BLAST-2，EBVCS）是一种45 kDa的谱系限制性抗原，在人B细胞激活后不久出现在其表面。已发现一种针对CD23，p45的单克隆抗体（MHM6）以及一种针对纯化抗原产生的兔多克隆抗体可促进用佛波酯激活的纯化扁桃体B细胞中的DNA合成。白细胞介素1本身对静止或激活的B细胞均无刺激作用，但能显著增强MHM6的促生长特性。动力学研究表明，MHM6在G1早期发挥作用，而白细胞介素1在细胞进入S期之前的细胞周期后期起作用。这些发现证明了CD23，p45在触发活化B淋巴细胞通过细胞周期G1期进程中的作用。本文讨论了该抗原作为B细胞刺激因子受体的可能性。
