Hassani-Mehraban A, Dullemans A M, Verhoeven J Th J, Roenhorst J W, Peters D, van der Vlugt R A A, Kormelink R
Wageningen University and Research, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands.
The National Plant Protection Organisation (NPPO) of the Netherlands, P.O. Box 9102, 6700 HC, Wageningen, The Netherlands.
Arch Virol. 2019 Jan;164(1):117-126. doi: 10.1007/s00705-018-4027-z. Epub 2018 Oct 4.
An orthotospovirus distinct from all other orthotospoviruses was isolated from naturally infected alstroemeria plants. Disease symptoms caused by this virus mainly consisted of yellow spots on the leaves based on which the name alstroemeria yellow spot virus (AYSV) was coined. A host range analysis was performed and a polyclonal antiserum was produced against purified AYSV ribonucleoproteins which only reacted with the homologous antigen and not with any other (established or tentative) orthotospovirus from a selection of American and Asian species. Upon thrips transmission assays the virus was successfully transmitted by a population of Thrips tabaci. The entire nucleotide sequence of the M and S RNA segments was elucidated by a conventional cloning and sequencing strategy, and contained 4797 respectively 2734 nucleotides (nt). Simultaneously, a next generation sequencing (NGS) approach (RNAseq) was employed and generated contigs covering the entire viral tripartite RNA genome. In addition to the M and S RNA nucleotide sequences, the L RNA (8865 nt) was obtained. The nucleocapsid (N) gene encoded by the S RNA of this virus consisted of 819 nucleotides with a deduced N protein of 272 amino acids and by comparative sequence alignments to other established orthotospovirus species showed highest homology (69.5% identity) to the N protein of polygonum ringspot virus. The data altogether support the proposal of AYSV as a new orthotospovirus species within a growing clade of orthotospoviruses that seem to share the Middle East basin as a region of origin.
从自然感染的六出花植株中分离出一种与所有其他番茄斑萎病毒属病毒不同的番茄斑萎病毒属病毒。这种病毒引起的病害症状主要表现为叶片上出现黄斑，据此命名为六出花黄斑病毒（AYSV）。进行了寄主范围分析，并针对纯化的AYSV核糖核蛋白制备了多克隆抗血清，该抗血清仅与同源抗原发生反应，而不与从一些美洲和亚洲物种中选取的任何其他（已确定或暂定的）番茄斑萎病毒属病毒发生反应。在蓟马传播试验中，该病毒成功地由烟蓟马群体传播。通过传统的克隆和测序策略阐明了M和S RNA片段的完整核苷酸序列，分别包含4797个和2734个核苷酸（nt）。同时，采用了下一代测序（NGS）方法（RNAseq），生成了覆盖整个病毒三方RNA基因组的重叠群。除了M和S RNA核苷酸序列外，还获得了L RNA（8865 nt）。该病毒S RNA编码的核衣壳（N）基因由819个核苷酸组成，推导的N蛋白有272个氨基酸，通过与其他已确定的番茄斑萎病毒属病毒物种进行比较序列比对，显示与虎杖环斑病毒的N蛋白具有最高同源性（69.5%同一性）。这些数据共同支持将AYSV提议为番茄斑萎病毒属病毒一个新物种，该属病毒似乎以中东盆地为起源区域，且种类不断增加。
