在大肠杆菌中表达两个干扰素-γ序列期间色氨酸操纵子的诱导

trp operon induction during the expression in E. coli of two IFN-gamma sequences.

作者信息

Sverdlov E D, Tsarev S A, Krykbaev R A, Chernov I P, Rostapshov V M

出版信息

FEBS Lett. 1987 Feb 23;212(2):233-6. doi: 10.1016/0014-5793(87)81351-0.

DOI:10.1016/0014-5793(87)81351-0

Abstract

Two nucleotide sequences coding for mature human immune interferon (IFN-gamma) and differing from each other by nine N-terminal nucleotides were expressed in E. coli under the control of a trp promoter. The longer gene variant after the ATG initiatory codon contained a TGT TAC TGC sequence, which was absent in the shorter gene. When expressed in E. coli under the direction of identical transcription and translation regulatory elements, these genes showed different susceptibility to induction.

摘要

两种编码成熟人免疫干扰素（IFN-γ）且在N端九个核苷酸上彼此不同的核苷酸序列，在色氨酸启动子的控制下于大肠杆菌中表达。起始密码子ATG之后较长的基因变体含有TGT TAC TGC序列，而较短的基因中没有该序列。当在相同的转录和翻译调控元件指导下于大肠杆菌中表达时，这些基因显示出不同的诱导敏感性。

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