小鼠免疫干扰素cDNA的克隆与表达
Cloning and expression of murine immune interferon cDNA.
作者信息
Gray P W, Goeddel D V
出版信息
Proc Natl Acad Sci U S A. 1983 Oct;80(19):5842-6. doi: 10.1073/pnas.80.19.5842.
Abstract
The murine immune interferon (IFN-gamma) gene was cloned and expressed under control of the simian virus 40 early promoter in the monkey COS-1 cell line. A protein is secreted from these cells having the biological, antigenic, and biochemical characteristics of natural murine IFN-gamma. Cloned murine IFN-gamma cDNAs were obtained by using RNA from both mitogen-induced murine spleens and the transfected COS cells, and both code for identical proteins. The mature murine IFN-gamma encoded is 136 amino acids long, 10 amino acids shorter than human IFN-gamma. The nucleotide homology between the murine and human IFN-gamma genes is 60-65%, whereas the encoded proteins are only 40% homologous. Murine IFN-gamma cDNA was expressed in Escherichia coli under trp promoter control.
摘要
鼠免疫干扰素（IFN-γ）基因在猴COS-1细胞系中，于猿猴病毒40早期启动子的控制下被克隆并表达。从这些细胞中分泌出一种具有天然鼠IFN-γ生物学、抗原性和生化特性的蛋白质。通过使用来自丝裂原诱导的鼠脾脏和转染的COS细胞的RNA获得了克隆的鼠IFN-γ cDNA，二者编码相同的蛋白质。所编码的成熟鼠IFN-γ长136个氨基酸，比人IFN-γ短10个氨基酸。鼠和人IFN-γ基因之间的核苷酸同源性为60%-65%，而所编码的蛋白质同源性仅为40%。鼠IFN-γ cDNA在色氨酸启动子的控制下在大肠杆菌中表达。
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