使用偏振敏感二次谐波产生显微镜对病理性胃组织进行表征。
Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy.
作者信息
Jeon Hwanhee, Harvey MacAulay, Cisek Richard, Bennett Elisha, Tokarz Danielle
机构信息
Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia, B3H 3C3, Canada.
出版信息
Biomed Opt Express. 2023 Sep 22;14(10):5376-5391. doi: 10.1364/BOE.500335. eCollection 2023 Oct 1.
Abstract
Alterations in collagen ultrastructure between human gastric adenocarcinoma and normal gastric tissue were investigated using polarization-resolved second harmonic generation (PSHG) microscopy. Cylindrical and trigonal symmetries were assumed to extract quantitative PSHG parameters, , and , from each image pixel. Statistically significant variations in these values were observed for gastric adenocarcinoma, indicating a higher disorder of collagen. Numerical focal volume simulations of crossing fibrils indicate increased parameter is due to more intersecting collagen fibrils of varying diameters. These parameters were also able to distinguish between different grades of gastric adenocarcinoma indicating that PSHG may be useful for automated cancer diagnosis.
摘要
利用偏振分辨二次谐波产生（PSHG）显微镜研究了人胃腺癌与正常胃组织之间胶原超微结构的变化。假设圆柱和三角对称性以从每个图像像素中提取定量的PSHG参数、 和 。观察到这些值在胃腺癌中存在统计学上的显著差异，表明胶原的无序程度更高。交叉纤维的数值焦体积模拟表明，参数增加是由于更多不同直径的交叉胶原纤维。这些参数还能够区分不同等级的胃腺癌，表明PSHG可能有助于自动癌症诊断。
相似文献
1
Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy.使用偏振敏感二次谐波产生显微镜对病理性胃组织进行表征。
Biomed Opt Express. 2023 Sep 22;14(10):5376-5391. doi: 10.1364/BOE.500335. eCollection 2023 Oct 1.
2
High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.高数值孔径成像允许使用偏振二次谐波产生显微镜对单个胶原纤维中的手性进行测量。
Nanophotonics. 2023 Apr 14;12(11):2061-2071. doi: 10.1515/nanoph-2023-0177. eCollection 2023 May.
3
PSHG-TISS: A collection of polarization-resolved second harmonic generation microscopy images of fixed tissues.PSHG-TISS：一组固定组织的偏振分辨二次谐波产生显微镜图像集合。
Sci Data. 2022 Jul 2;9(1):376. doi: 10.1038/s41597-022-01477-1.
4
Comparison of Different Polarization Sensitive Second Harmonic Generation Imaging Techniques.不同偏振敏感二次谐波产生成像技术的比较
Methods Protoc. 2019 Jun 7;2(2):49. doi: 10.3390/mps2020049.
5
Polarization-dependent second-harmonic generation for collagen-based differentiation of breast cancer samples.基于偏振相关性二次谐波产生的乳腺癌样本胶原差异分化
J Biophotonics. 2020 Oct;13(10):e202000180. doi: 10.1002/jbio.202000180. Epub 2020 Aug 12.
6
Fast polarization-sensitive second-harmonic generation microscopy based on off-axis interferometry.基于离轴干涉的快速偏振敏感二次谐波产生显微镜。
Opt Express. 2023 Jan 16;31(2):3143-3152. doi: 10.1364/OE.471459.
7
Polarization-resolved super-resolution second-harmonic generation imaging based on multifocal structured illumination microscopy.基于多焦点结构照明显微术的偏振分辨超分辨率二次谐波生成成像
Opt Lett. 2024 Mar 15;49(6):1540-1543. doi: 10.1364/OL.514724.
8
Microscopic structural study of collagen aging in isolated fibrils using polarized second harmonic generation.利用偏振二次谐波产生研究分离纤维中胶原蛋白的老化的微观结构。
J Biomed Opt. 2012 Aug;17(8):080506-1. doi: 10.1117/1.JBO.17.8.080506.
9
Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils.平面外取向对单根胶原纤维偏振二次谐波产生的影响。
Biomed Opt Express. 2023 Nov 13;14(12):6271-6282. doi: 10.1364/BOE.504304. eCollection 2023 Dec 1.
10
Influence of hematoxylin and eosin staining on the quantitative analysis of second harmonic generation imaging of fixed tissue sections.苏木精和伊红染色对固定组织切片二次谐波产生成像定量分析的影响
Biomed Opt Express. 2021 Aug 25;12(9):5829-5843. doi: 10.1364/BOE.428701. eCollection 2021 Sep 1.
引用本文的文献
1
determination and matching of the refractive index of the human cornea to improve polarization-resolved SHG imaging in depth.确定并匹配人眼角膜的折射率以改善深度分辨偏振分辨二次谐波成像。
Biomed Opt Express. 2025 Jul 18;16(8):3270-3282. doi: 10.1364/BOE.564209. eCollection 2025 Aug 1.
2
Spatial polarimetric second harmonic generation evaluation of collagen in a hypophosphatasia mouse model.低磷酸酯酶症小鼠模型中胶原蛋白的空间偏振二次谐波产生评估
Biomed Opt Express. 2024 Nov 22;15(12):6940-6956. doi: 10.1364/BOE.529428. eCollection 2024 Dec 1.
3
Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils.平面外取向对单根胶原纤维偏振二次谐波产生的影响。
Biomed Opt Express. 2023 Nov 13;14(12):6271-6282. doi: 10.1364/BOE.504304. eCollection 2023 Dec 1.
本文引用的文献
1
High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.高数值孔径成像允许使用偏振二次谐波产生显微镜对单个胶原纤维中的手性进行测量。
Nanophotonics. 2023 Apr 14;12(11):2061-2071. doi: 10.1515/nanoph-2023-0177. eCollection 2023 May.
2
Investigation into the structure of crystalline maltodextrin particles by second harmonic generation microscopy.利用二次谐波产生显微镜对结晶麦芽糊精颗粒的结构进行研究。
Biomed Opt Express. 2023 Feb 7;14(3):1027-1040. doi: 10.1364/BOE.481689. eCollection 2023 Mar 1.
3
Second harmonic generation microscopy of otoconia.耳石的二次谐波产生显微镜检查
Biomed Opt Express. 2022 May 26;13(6):3593-3600. doi: 10.1364/BOE.457967. eCollection 2022 Jun 1.
4
The current and future incidence and mortality of gastric cancer in 185 countries, 2020-40: A population-based modelling study.2020 - 2040年185个国家胃癌的当前及未来发病率和死亡率：一项基于人群的建模研究
EClinicalMedicine. 2022 Apr 21;47:101404. doi: 10.1016/j.eclinm.2022.101404. eCollection 2022 May.
5
Evaluation of Collagen Alterations in Early Precursor Lesions of High Grade Serous Ovarian Cancer by Second Harmonic Generation Microscopy and Mass Spectrometry.通过二次谐波产生显微镜和质谱法评估高级别浆液性卵巢癌早期前体病变中的胶原蛋白变化
Cancers (Basel). 2021 Jun 4;13(11):2794. doi: 10.3390/cancers13112794.
6
Current treatment and recent progress in gastric cancer.胃癌的当前治疗方法和最新进展。
CA Cancer J Clin. 2021 May;71(3):264-279. doi: 10.3322/caac.21657. Epub 2021 Feb 16.
7
A quantitative and qualitative comparative analysis of collagen fibers to determine the role of connective tissue stroma in oral squamous cell carcinoma using special stains and polarized microscopy.使用特殊染色和偏振显微镜对胶原纤维进行定量和定性比较分析，以确定结缔组织基质在口腔鳞状细胞癌中的作用。
J Oral Maxillofac Pathol. 2020 May-Aug;24(2):398. doi: 10.4103/jomfp.JOMFP_84_18. Epub 2020 Sep 9.
8
Objective analysis of collagen organization in thyroid nodule capsules using second harmonic generation microscopy images and the Hough transform.利用二次谐波产生显微镜图像和霍夫变换对甲状腺结节囊胶原组织进行客观分析。
Appl Opt. 2020 Aug 10;59(23):6925-6931. doi: 10.1364/AO.393721.
9
Characterization of pathological thyroid tissue using polarization-sensitive second harmonic generation microscopy.应用偏振敏感二次谐波产生显微镜对病态甲状腺组织进行特征分析。
Lab Invest. 2020 Oct;100(10):1280-1287. doi: 10.1038/s41374-020-0475-7. Epub 2020 Jul 31.
10
Polarization-dependent second-harmonic generation for collagen-based differentiation of breast cancer samples.基于偏振相关性二次谐波产生的乳腺癌样本胶原差异分化
J Biophotonics. 2020 Oct;13(10):e202000180. doi: 10.1002/jbio.202000180. Epub 2020 Aug 12.
Zotero 插件