Jeon Hwanhee, Harvey MacAulay, Cisek Richard, Bennett Elisha, Tokarz Danielle
Department of Chemistry, Saint Mary's University, 923 Robie Street, Halifax, Nova Scotia, B3H 3C3, Canada.
Biomed Opt Express. 2023 Sep 22;14(10):5376-5391. doi: 10.1364/BOE.500335. eCollection 2023 Oct 1.
Alterations in collagen ultrastructure between human gastric adenocarcinoma and normal gastric tissue were investigated using polarization-resolved second harmonic generation (PSHG) microscopy. Cylindrical and trigonal symmetries were assumed to extract quantitative PSHG parameters, , and , from each image pixel. Statistically significant variations in these values were observed for gastric adenocarcinoma, indicating a higher disorder of collagen. Numerical focal volume simulations of crossing fibrils indicate increased parameter is due to more intersecting collagen fibrils of varying diameters. These parameters were also able to distinguish between different grades of gastric adenocarcinoma indicating that PSHG may be useful for automated cancer diagnosis.
利用偏振分辨二次谐波产生（PSHG）显微镜研究了人胃腺癌与正常胃组织之间胶原超微结构的变化。假设圆柱和三角对称性以从每个图像像素中提取定量的PSHG参数、 和 。观察到这些值在胃腺癌中存在统计学上的显著差异，表明胶原的无序程度更高。交叉纤维的数值焦体积模拟表明，参数增加是由于更多不同直径的交叉胶原纤维。这些参数还能够区分不同等级的胃腺癌，表明PSHG可能有助于自动癌症诊断。
