Yoon Jeongmin, Yoon Eul-Sik, Lee Byung-Il, Park Seung-Ha, Kim Jin Woo
a Department of Plastic and Reconstructive Surgery , Korea University Anam Hospital, Korea University College of Medicine , Seoul , Republic of Korea.
J Plast Surg Hand Surg. 2019 Feb;53(1):25-30. doi: 10.1080/2000656X.2018.1523179. Epub 2018 Oct 5.
Botulinum toxin has been recently known for its vasodilatory effect. The main purpose of our study was to demonstrate the anti-vasospastic effect of botulinum toxin B in animal models.
In this prospective experimental rat study, we used 10 male Sprague-Dawley rats. The right and left inguinal areas (femoral vessels) served as the experimental and control groups, respectively. Three days prior to the experimental procedure, botulinum toxin B and normal saline were subcutaneously injected in the experimental and control groups, respectively. After three days, the vascular diameter and flow were measured. Then, phenylephrine was injected, and after one hour the same parameters were measured.
The changes in arterial diameter in the experimental and control group were 30.83 ± 10.43% and 40.00 ± 8.61%, respectively. The vein diameter decreased by 22.98 ± 10.08% in the experimental group compared to 49.05 ± 10.08% for the control group. Furthermore, the arterial flow change in the control group was 8.28 ± 2.10% compared to 1.16 ± 0.65% in the experimental group. In addition, the venous flow in the control group decreased by 9.27 ± 2.75% compared to 0.92 ± 0.56% in the experimental group. Both arterial and venous diameters and flows showed statistical significance between the experimental and control groups (p < .05).
Our study demonstrated that pretreatment with botulinum toxin B led to significantly reduced change in vascular diameters and flows compared with the control group, supporting the anti-vasospastic effects of botulinum toxin B.
肉毒杆菌毒素最近因其血管舒张作用而为人所知。我们研究的主要目的是在动物模型中证明肉毒杆菌毒素B的抗血管痉挛作用。
在这项前瞻性实验性大鼠研究中，我们使用了10只雄性Sprague-Dawley大鼠。左右腹股沟区域（股血管）分别作为实验组和对照组。在实验程序前三天，分别向实验组和对照组皮下注射肉毒杆菌毒素B和生理盐水。三天后，测量血管直径和血流量。然后注射去氧肾上腺素，一小时后测量相同参数。
实验组和对照组的动脉直径变化分别为30.83±10.43%和40.00±8.61%。实验组静脉直径下降了22.98±10.08%，而对照组为49.05±10.08%。此外，对照组的动脉血流变化为8.28±2.10%，而实验组为1.16±0.65%。另外，对照组的静脉血流下降了9.27±2.75%，而实验组为0.92±0.56%。实验组和对照组之间的动脉和静脉直径及血流均显示出统计学意义（p<0.05）。
我们的研究表明，与对照组相比，肉毒杆菌毒素B预处理导致血管直径和血流变化显著减少，支持了肉毒杆菌毒素B的抗血管痉挛作用。
