Filipe J, Curone G, Bronzo V, Pisoni G, Cremonesi P, Pollera C, Turin L, Vigo D, Roccabianca P, Caniatti M, Moroni P, Riva F
Università degli Studi di Milano, Dipartimento di Medicina Veterinaria, Via Celoria 10, 20133 Milan, Italy.
Veterinary Practitioner, Italy.
Comp Immunol Microbiol Infect Dis. 2018 Aug;59:8-16. doi: 10.1016/j.cimid.2018.08.007. Epub 2018 Sep 11.
Pentraxin 3 is the prototypic long pentraxin and is produced by different cell populations (dendritic cells, monocytes/macrophages, endothelial cells, and fibroblasts) after pro-inflammatory stimulation. Different studies demonstrated the up-regulation of PTX3 during mastitis in ruminants, but its role is still unknown. We first investigated the conservation of PTX3 sequence among different species and its pattern of expression in a wide panel of organs from healthy goats. We studied the modulation of PTX3 during natural and experimental mammary infection, comparing its expression in blood, milk and mammary tissues from healthy and Staphylococcus aureus infected animals. We confirmed the high conservation of the molecule among different species. Goat PTX3 was expressed at high levels in bone marrow, mammary gland, aorta, rectum, pancreas, skin and lungs. PTX3 was up-regulated in epithelial mammary cells and in milk cells after S. aureus infection, suggesting that it represents a first line of defense in goat udder.
五聚体蛋白3是典型的长链五聚体蛋白，由不同细胞群体（树突状细胞、单核细胞/巨噬细胞、内皮细胞和成纤维细胞）在促炎刺激后产生。不同研究表明反刍动物乳腺炎期间PTX3上调，但其作用仍不清楚。我们首先研究了PTX3序列在不同物种间的保守性及其在健康山羊多种器官中的表达模式。我们研究了自然和实验性乳腺感染期间PTX3的调节，比较了健康动物和金黄色葡萄球菌感染动物血液、乳汁和乳腺组织中PTX3的表达。我们证实了该分子在不同物种间具有高度保守性。山羊PTX3在骨髓、乳腺、主动脉、直肠、胰腺、皮肤和肺中高水平表达。金黄色葡萄球菌感染后，上皮乳腺细胞和乳汁细胞中PTX3上调，表明它是山羊乳房的第一道防线。
