Università degli Studi di Milano, Dipartimento di Medicina Veterinaria, via Celoria 10, 20133, Milan, Italy.
Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 26900, Lodi, Italy.
Comp Immunol Microbiol Infect Dis. 2019 Oct;66:101339. doi: 10.1016/j.cimid.2019.101339. Epub 2019 Jul 30.
IL-1R8 is a member of Interleukin-1 receptor family acting as a negative regulator of inflammation reliant on ILRs and TLRs activation. IL-1R8 role has never been evaluated in acute bacterial mastitis. We first investigated IL-1R8 sequence conservation among different species and its pattern of expression in a wide panel of organs from healthy goats. Then, modulation of IL-1R8 during natural and experimental mammary infection was evaluated and compared in blood, milk and mammary tissues from healthy and Staphylococcus aureus infected goats. IL-1R8 has a highly conserved sequence among vertebrates. Goat IL-1R8 was ubiquitously expressed in epithelial and lymphoid tissues with highest levels in pancreas. IL-1R8 was down-regulated in epithelial mammary cells following S. aureus infection. Interestingly it was up-regulated in leukocytes infiltrating the infected mammary tissues suggesting that it could represent a target of S. aureus immune evasion.
IL-1R8 是白细胞介素 1 受体家族的一员，作为炎症的负调节剂，依赖于 ILRs 和 TLRs 的激活。IL-1R8 在急性细菌性乳腺炎中的作用从未被评估过。我们首先研究了不同物种之间 IL-1R8 序列的保守性及其在健康山羊广泛器官中的表达模式。然后，在健康和金黄色葡萄球菌感染的山羊的血液、牛奶和乳腺组织中评估并比较了 IL-1R8 在自然和实验性乳腺感染期间的调节。IL-1R8 在脊椎动物中具有高度保守的序列。山羊的 IL-1R8 在上皮组织和淋巴组织中广泛表达，在胰腺中的表达水平最高。IL-1R8 在金黄色葡萄球菌感染后上皮乳腺细胞中下调。有趣的是，它在上皮细胞浸润的感染乳腺组织中的白细胞中上调，这表明它可能是金黄色葡萄球菌免疫逃避的一个靶点。
