Tsai T F, Bolin R A, Montoya M, Bailey R E, Francy D B, Jozan M, Roehrig J T
J Clin Microbiol. 1987 Feb;25(2):370-6. doi: 10.1128/jcm.25.2.370-376.1987.
Surveillance methods that measure St. Louis encephalitis (SLE) virus activity in nature may provide forewarning of its epidemic occurrence in humans. An antigen capture enzyme immunoassay was developed to detect SLE virus in infected mosquitoes. The assay detected purified SLE viral antigen at a concentration of 62 pg/0.1 ml when antigen was incubated overnight; 250 pg/0.1 ml was detected in a single-day assay (antigen incubated for 3 h). The assay detected 67.9 and 70.8% of laboratory-prepared pools of infected mosquitoes after 3 h and overnight incubation, respectively. The sensitivity of the procedure was 90.5% in identifying pools with infectious titers greater than dex 3.0. The specificity of the assay was controlled by retesting positive pools preincubated with SLE virus and normal antibodies, which led to a diminution of signal in the pools containing viral antigen. The procedure was suitably specific in discriminating between SLE and related flaviviruses, detecting only high infectious doses of heterologous antigens.
监测自然界中圣路易斯脑炎（SLE）病毒活动的方法可能会为该病毒在人类中的流行发生提供预警。已开发出一种抗原捕获酶免疫测定法来检测受感染蚊子中的SLE病毒。当抗原孵育过夜时，该测定法能检测到浓度为62 pg/0.1 ml的纯化SLE病毒抗原；在单日测定法（抗原孵育3小时）中检测到的浓度为250 pg/0.1 ml。该测定法在孵育3小时和过夜后，分别检测出实验室制备的受感染蚊子样本池的67.9%和70.8%。该方法在识别感染滴度大于对数3.0的样本池时灵敏度为90.5%。通过对预先与SLE病毒和正常抗体孵育的阳性样本池进行重新检测来控制该测定法的特异性，这导致含有病毒抗原的样本池中信号减弱。该方法在区分SLE和相关黄病毒方面具有适当的特异性，仅能检测到高感染剂量的异源抗原。
