Universidade do Estado da Bahia, Rua Silveira Martins, 2555, Cabula, Salvador, Bahia 41195-001, Brazil.
Universidade do Estado da Bahia, Rua Silveira Martins, 2555, Cabula, Salvador, Bahia 41195-001, Brazil; Universidade Federal da Bahia, Instituto de Química, Campus Ondina, Salvador, Bahia 40170-290, Brazil.
Food Chem. 2019 Feb 1;273:24-30. doi: 10.1016/j.foodchem.2018.05.020. Epub 2018 May 3.
In this work, an analytical method was proposed for the simultaneous determination of mercury and selenium in fish samples using Atomic Fluorescence Spectrometry (AFS). Multivariate designs were performed to evaluate the variables and optimize the best condition of chemical vapor generation (CVG) and simultaneous determination of mercury and selenium by AFS. Fish samples were prepared via acid digestion in digester block with cold finger reflux system, which ensured that the elements were not lost by volatility. The proposed analytical method was validated, and excellent figures of merit have been achieved, such as detection limits of 0.33 and 9.18 ng g for mercury and selenium, respectively. The method was applied for simultaneous determination of mercury and selenium in canned sardines. Mercury concentrations ranged from 0.057 to 0.203 μg g and selenium concentration from 1.76 to 2.21 μg g, providing a mean molar ratio (Se:Hg) equivalent to 36.
在这项工作中，提出了一种使用原子荧光光谱法（AFS）同时测定鱼样中汞和硒的分析方法。采用多元设计来评估变量，并优化 CVG 的最佳条件和 AFS 同时测定汞和硒的最佳条件。鱼样通过冷指回流系统的消解器块中的酸消解进行制备，以确保元素不会因挥发而损失。该分析方法经过验证，取得了优异的度量指标，例如汞和硒的检测限分别为 0.33 和 9.18ng/g。该方法应用于罐头沙丁鱼中汞和硒的同时测定。汞浓度范围为 0.057 至 0.203μg/g，硒浓度范围为 1.76 至 2.21μg/g，提供了一个平均摩尔比（Se:Hg），相当于 36。
