Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Jinan, Shandong, 250100, China.
Fish Shellfish Immunol. 2019 Jan;84:244-251. doi: 10.1016/j.fsi.2018.10.006. Epub 2018 Oct 4.
Thymosins β are actin-binding proteins that play a variety of different functions in inflammatory responses, wound healing, cell migration, angiogenesis, and stem cell recruitment and differentiation. In crayfish, thymosins participate in antiviral immunology. However, the roles of thymosin during bacterial infection in shrimp remain unclear. In the present study, four thymosins were identified from kuruma shrimp, Marsupenaeus japonicus, and named as Mjthymosin2, Mjthymosin3, Mjthymosin4, and Mjthymosin5 according the number of their thymosin beta actin-binding motifs. Mjthymosin3 was selected for further study because its expression level was the highest in hemocytes. Expression analysis showed that Mjthymosin3 was upregulated in hemocytes after challenged by Vibrio anguillarum or Staphylococcus aureus. The recombinant Mjthymosin3 protein could inhibit the growth of certain bacteria in an in vitro antibacterial test. Mjthymosins could facilitate external bacterial clearance in shrimp, and were beneficial to shrimp survival post V. anguillarum or S. aureus infection. The results suggested that Mjthymosins played important roles in the antibacterial immune response of kuruma shrimp.
β-胸腺素是一种肌动蛋白结合蛋白，在炎症反应、伤口愈合、细胞迁移、血管生成以及干细胞募集和分化等多种不同的功能中发挥作用。在螯虾中，β-胸腺素参与抗病毒免疫。然而，在虾类的细菌感染过程中，β-胸腺素的作用尚不清楚。在本研究中，从日本对虾中鉴定了 4 种β-胸腺素，并根据其β-actin 结合基序的数量分别命名为 Mjthymosin2、Mjthymosin3、Mjthymosin4 和 Mjthymosin5。选择 Mjthymosin3 进行进一步研究，因为它在血细胞中的表达水平最高。表达分析表明，在受到鳗弧菌或金黄色葡萄球菌攻击后，血细胞中的 Mjthymosin3 表达上调。重组 Mjthymosin3 蛋白在体外抗菌试验中可抑制某些细菌的生长。在虾类中，β-胸腺素可以促进外来细菌的清除，有利于虾类在感染鳗弧菌或金黄色葡萄球菌后的存活。这些结果表明，β-胸腺素在日本对虾的抗菌免疫反应中发挥了重要作用。
