Arias C F, Lizano M, López S
J Gen Virol. 1987 Mar;68 ( Pt 3):633-42. doi: 10.1099/0022-1317-68-3-633.
About 45% of the rotavirus SA11 VP3 gene was inserted into a thermoinducible expression plasmid under the control of phage lambda PL promoter. The primary translation product predicted on the basis of the plasmid construction was a hybrid protein in which the 98 amino-terminal amino acids of phage MS2 polymerase were followed by amino acids 42 to 387 of the VP3 protein, which included the region containing the cleavage sites associated with trypsin enhancement of infectivity. On induction, a polypeptide that had the expected mol. wt. and contained VP3-related amino acid sequences as judged by immunological criteria, was synthesized to a level representing about 15% of the total bacterial protein. When a bacterial lysate enriched for the fusion polypeptide was injected into mice, it induced antibodies which inhibited haemagglutination and neutralized SA11 rotavirus infectivity.
约45%的轮状病毒SA11 VP3基因被插入到一个受噬菌体λ PL启动子控制的热诱导表达质粒中。根据质粒构建预测的初级翻译产物是一种杂合蛋白，其中噬菌体MS2聚合酶的98个氨基末端氨基酸后面跟着VP3蛋白的42至387个氨基酸，这包括含有与胰蛋白酶增强感染性相关切割位点的区域。诱导后，合成了一种具有预期分子量且根据免疫学标准含有VP3相关氨基酸序列的多肽，其水平约占细菌总蛋白的15%。当将富含融合多肽的细菌裂解物注射到小鼠体内时，它诱导产生了抑制血凝和中和SA11轮状病毒感染性的抗体。
