Kantharidis P, Dyall-Smith M L, Tregear G W, Holmes I H
Department of Microbiology, University of Melbourne, Parkville, Victoria, Australia.
Virology. 1988 Oct;166(2):308-15. doi: 10.1016/0042-6822(88)90501-6.
The bovine UK and simian SA11 rotaviruses are commonly used VP7-type reference strains. Since the surface protein VP3 is a significant neutralization antigen, it is important to fully characterize the VP3 types associated with current reference strains. Here we present the complete nucleotide and predicted amino acid sequence of VP3 from UK rotavirus (VP7 type 6) and compare it to the published sequences of SA114fm and RV-5. We also compare the deduced amino acid sequence covering the trypsin cleavage region of UK VP3 to 25 other available sequences. The UK protein is clearly different from that of bovine NCDV (another commonly used VP7 type 6 strain) and represents a second VP3 type associated with bovine rotaviruses. Our SA11 sequence differs from that determined by Lopez et al. [1985, Virology 144, 11-19; later referred to as SA114fM by Lopez et al. (1986, Virology 154, 224-227], their sequence being very similar to the published sequence of NCDV VP3. The significance of these results with regard to virus serotypes is discussed. Finally, in analyzing the nucleotide sequence surrounding the initiation codon, a potential hairpin-loop structure was identified which may be involved in translational regulation.
牛源英国株和猿猴源SA11轮状病毒是常用的VP7型参考毒株。由于表面蛋白VP3是一种重要的中和抗原，全面鉴定与当前参考毒株相关的VP3类型很重要。在此，我们展示了英国轮状病毒（VP7 6型）VP3的完整核苷酸序列和预测的氨基酸序列，并将其与已发表的SA114fm和RV-5序列进行比较。我们还将英国VP3胰蛋白酶切割区域的推导氨基酸序列与其他25个可用序列进行了比较。英国株的蛋白明显不同于牛源NCDV（另一种常用的VP7 6型毒株），代表了与牛轮状病毒相关的第二种VP3类型。我们的SA11序列与Lopez等人[1985年，《病毒学》144卷，第11 - 19页；后来Lopez等人（1986年，《病毒学》154卷，第224 - 227页）将其称为SA114fM]确定的序列不同，他们的序列与已发表的NCDV VP3序列非常相似。讨论了这些结果在病毒血清型方面的意义。最后，在分析起始密码子周围的核苷酸序列时，鉴定出一个潜在的发夹环结构，它可能参与翻译调控。
