School of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar, Heilongjiang, 161006, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Ecotoxicol Environ Saf. 2019 Jan 15;167:36-43. doi: 10.1016/j.ecoenv.2018.09.078. Epub 2018 Oct 4.
Dimethyl phthalate (DMP) is a ubiquitous pollutant that is very harmful to organisms due to its mutagenicity, teratogenicity and carcinogenicity. Pseudomonas fluorescens (P. fluorescens) is one of the most important bacteria in the environment. In this study, the response of P. fluorescens to DMP was investigated. It was found that DMP greatly inhibited the growth and glucose utilization of P. fluorescens when the concentration of DMP was ranged from 20 to 40 mg/l. The surface hydrophobicity and membrane permeability of P. fluorescens were also increased by DMP. DMP could lead to the deformations of cell membrane and the mis-opening of membrane channels. RNA-Seq and RT-qPCR results showed that the expression of some genes in P. fluorescens were altered, including the genes involved in energy metabolism, ATP-binding cassette (ABC) transporting and two-component systems. Additionally, the productions of lactic acid and pyruvic acid were reduced and the activity of hexokinase was inhibited in P. fluorescens by DMP. Clearly, the results suggested that DMP contamination could alter the biological function of P. fluorescens in the environment.
邻苯二甲酸二甲酯（DMP）是一种普遍存在的污染物，由于其具有致突变性、致畸性和致癌性，对生物体非常有害。荧光假单胞菌（P. fluorescens）是环境中最重要的细菌之一。在这项研究中，研究了 P. fluorescens 对 DMP 的反应。结果发现，当 DMP 浓度在 20 至 40mg/L 范围内时，DMP 会极大地抑制 P. fluorescens 的生长和葡萄糖利用。DMP 还增加了 P. fluorescens 的表面疏水性和膜通透性。DMP 可导致细胞膜变形和膜通道错误打开。RNA-Seq 和 RT-qPCR 结果表明，P. fluorescens 中的一些基因的表达发生了改变，包括参与能量代谢、ABC 转运和双组分系统的基因。此外，DMP 降低了 P. fluorescens 中乳酸和丙酮酸的产量，并抑制了己糖激酶的活性。显然，这些结果表明 DMP 污染可能会改变 P. fluorescens 在环境中的生物功能。
