Linde A, Andersson J, Lundgren G, Wahren B
J Med Virol. 1987 Feb;21(2):109-21. doi: 10.1002/jmv.1890210203.
A new method for analysis of virus-specific Immunoglobulin G (IgG) subclasses was developed using indirect immunofluorescence. Three hundred thirty-three serum samples from patients with different types of Epstein-Barr virus (EBV)-associated diseases and healthy controls were examined for subclass distribution to the virus capsid antigen (EBV VCA). EBV-VCA-expressing cell preparations were incubated with patient serum followed by monoclonal antibodies to human IgG1 through IgG4 and labelled anti-mouse IgG. Virus-specific IgG1 was found to be the dominant antibody. The titers for IgG1 and total Ig to EBV VCA correlated well. EBV VCA-specific IgG2 was not found. EBV VCA-specific IgG3 in a titer of greater than or equal to 10 was found in 33% of healthy seropositive donors, in 97% of patients with suspected reactivated EBV infection, and in 100% of symptomatic patients with suspected reactivated EBV infection. EBV VCA specific IgG3 occurred in 90% of placebo-treated compared to 30% in long-term acyclovir-treated bone marrow transplant recipients, indicating more frequent reactivations in the former group. IgG4 to VCA was infrequently found in seropositive persons. In serum samples from patients with nasopharyngeal carcinoma and high EBV VCA Ig and IgA titers, IgG4 to VCA was always present. Analysis of EBV VCA specific IgG subclasses seems to be valuable for the diagnosis of reactivated EBV infection.
利用间接免疫荧光技术建立了一种分析病毒特异性免疫球蛋白G（IgG）亚类的新方法。对333份来自不同类型爱泼斯坦-巴尔病毒（EBV）相关疾病患者及健康对照者的血清样本进行检测，分析其针对病毒衣壳抗原（EBV VCA）的亚类分布情况。将表达EBV-VCA的细胞制剂与患者血清孵育，然后依次加入针对人IgG1至IgG4的单克隆抗体以及标记的抗小鼠IgG。发现病毒特异性IgG1是主要抗体。IgG1和针对EBV VCA的总Ig滴度具有良好的相关性。未检测到EBV VCA特异性IgG2。在33%的健康血清阳性供者、97%疑似EBV再激活感染患者以及100%有症状的疑似EBV再激活感染患者中，发现EBV VCA特异性IgG3滴度大于或等于10。在接受安慰剂治疗的患者中，90%出现EBV VCA特异性IgG3，而在接受长期阿昔洛韦治疗的骨髓移植受者中这一比例为30%，表明前一组的再激活更为频繁。血清阳性者中很少检测到针对VCA的IgG4。在鼻咽癌患者且EBV VCA Ig和IgA滴度高的血清样本中，总是存在针对VCA的IgG4。分析EBV VCA特异性IgG亚类似乎对诊断再激活的EBV感染具有重要价值。
