Qin L, Wang Z L, Feng Y, Chen Z Z, Yu H
Graduate School of Baotou Medical College, Baotou 014060, China.
Zhonghua Xin Xue Guan Bing Za Zhi. 2018 Sep 24;46(9):706-712. doi: 10.3760/cma.j.issn.0253-3758.2018.09.007.
To characterize the gut microbial community structure of two-kidney-one-clip (2K1C) hypertensive rats, provide new evidences for prevention and treatment of hypertension. Adult male SD rats were divided into 2K1C hypertensive model group and sham operation group (8 each). Blood pressure was recorded by tail-cuff technique weekly for 4 weeks. Then, the total fecal DNA of rats in 2K1C model group and sham operation group were extracted. The structure and principal components of intestinal flora in 2K1C model rats and sham operated rats were analyzed using Hiseq3000 sequencing platform. Additionally, real time fluorescent quantitative polymerase chain reaction technology was used to detect the content of predominant bacteria, including Escherichia, Bifidobacterium, Lactobacillus and Bacteroides. Compared with sham operation group, significant reduction in the relative abundance of Firmicutes (31.72 (30.06, 32.18) vs. 40.99 (38.78, 44.41), =0.00, 0.05) and increase in Bacteroidetes (48.13(41.16,50.25) vs. 27.81(25.84,31.38),=0.00, 0.05) were observed in the fecal samples from model group. The relative abundance of Escherichia coli (0.08(0.07,0.11) vs. 0.07(0.06,0.08),=12.50, 0.05) was increased and the short chain fatty acid producing strains such as Coprococcus, Roseburia, Blautia, Clostridium and Bacteroides was reduced in 2K1C model group than in sham operation group (0.05). Moreover, the relative abundance of Prevotella (Bacteroidetes) was also significantly higher in 2K1C model group than in sham operation group (18.14(17.78,18.75) vs.1.83(1.50,5.19), =0.00, 0.05). Moreover, the relative abundance of Ruminococcus (Firmicutes) was significant higher in 2K1C model group than in sham operation group (7.73(6.04,9.34) vs. 3.68(2.46,4.67),=0.00, 0.05). Principal coordinate analysis showed that there was significant difference in the gut microbial community structure between 2K1C model group and sham operation group. There are significant changes in the gut microbial community structure in 2K1C model group as compared to sham operation group, indicating renovascular hypertension might affect gut microbial community structure in this rat model.
为了表征两肾一夹（2K1C）高血压大鼠的肠道微生物群落结构，为高血压的防治提供新的证据。将成年雄性SD大鼠分为2K1C高血压模型组和假手术组（每组8只）。采用尾套法每周记录血压，共4周。然后，提取2K1C模型组和假手术组大鼠的粪便总DNA。利用Hiseq3000测序平台分析2K1C模型大鼠和假手术大鼠肠道菌群的结构和主要成分。此外，采用实时荧光定量聚合酶链反应技术检测主要细菌的含量，包括大肠杆菌、双歧杆菌、乳酸杆菌和拟杆菌。与假手术组相比，模型组粪便样本中厚壁菌门的相对丰度显著降低（31.72（30.06，32.18）对40.99（38.78，44.41），P = 0.00，<0.05），拟杆菌门的相对丰度增加（48.13（41.16，50.25）对27.81（25.84，31.38），P = 0.00，<0.05）。2K1C模型组大肠杆菌的相对丰度增加（0.08（0.07，0.11）对0.07（0.06，0.08），P = 12.50，<0.05），而产短链脂肪酸的菌株如粪球菌、罗氏菌、布劳特氏菌、梭菌和拟杆菌在2K1C模型组中比假手术组减少（P<0.05）。此外，2K1C模型组普雷沃氏菌（拟杆菌门）的相对丰度也显著高于假手术组（18.14（17.78，18.75）对1.83（1.50，5.19），P = 0.00，<0.05）。此外，2K1C模型组瘤胃球菌（厚壁菌门）的相对丰度显著高于假手术组（7.73（6.04，9.34）对3.68（2.46，4.67），P = 0.00，<0.05）。主坐标分析表明，2K1C模型组和假手术组的肠道微生物群落结构存在显著差异。与假手术组相比，2K1C模型组的肠道微生物群落结构有显著变化，表明肾血管性高血压可能影响该大鼠模型的肠道微生物群落结构。
