Laboratory of Cellular and Molecular Immunology, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil; Research Center, Methodist University Center IPA, Porto Alegre, RS 90420-060, Brazil.
Laboratory of Cellular and Molecular Immunology, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS 90050-170, Brazil.
Prostaglandins Leukot Essent Fatty Acids. 2018 Oct;137:5-11. doi: 10.1016/j.plefa.2018.07.017. Epub 2018 Jul 24.
To evaluate the effects of acute fish oil supplementation (FOS) in DNA damage, lymphocyte phenotype and cytokines production after strenuous exercise in obese individuals.
Sixteen sedentary obese (BMI >30.0 to <35.0 kg/m²) men performed two sessions of exhaustive exercise and consumed 2000 mg of either placebo or fish oil one hour before the exercise session; trials were separated by 14 days. Peripheral blood mononuclear cells were collected pre, immediately after and 1 h after both exercise sessions and stimulated in vitro with 2% phytohemagglutinin for cytokines secretion (IL-6, IL-8, TNF-α). Analysis of DNA damage index on total lymphocytes and the peripheral frequency of T helper CD4+ cells, T cytotoxic CD8+ cells, and CD19+ B cells were also performed.
FOS prevented the increase in serum cortisol levels and the production of TNF-α and IL-8 after strenuous exercise. The DNA damage index decreased 1 h after exercise in FOS trial. Moreover, a lymphocytosis, i.e. increases in the frequency of CD4+ and CD8+ T cells was observed immediately after exercise bout in both trials. Moreover, FOS prevented the decrease in CD8+ T cells below to baseline value 1 h after strenuous exercise.
Acute supplementation with fish oil attenuates the proinflammatory cytokine response and diminished the DNA damage after strenuous exercise in obese individuals, suggesting a possible protective effect against the exacerbation of systemic damage induced by exhaustive exercise in obese individuals.
评估急性鱼油补充（FOS）对肥胖个体剧烈运动后 DNA 损伤、淋巴细胞表型和细胞因子产生的影响。
16 名久坐肥胖（BMI>30.0 至<35.0kg/m²）男性进行了两次耗竭性运动，并在运动前 1 小时分别服用 2000mg 安慰剂或鱼油；两次试验间隔 14 天。在两次运动后立即收集外周血单核细胞，并在体外用 2%植物血凝素刺激细胞因子分泌（IL-6、IL-8、TNF-α）。还分析了总淋巴细胞的 DNA 损伤指数以及 T 辅助 CD4+细胞、T 细胞毒性 CD8+细胞和 CD19+B 细胞的外周频率。
FOS 可防止剧烈运动后血清皮质醇水平和 TNF-α、IL-8 的产生增加。FOS 试验中，运动后 1 小时 DNA 损伤指数降低。此外，在两次试验中，运动后立即观察到淋巴细胞增多，即 CD4+和 CD8+T 细胞频率增加。此外，FOS 可防止 CD8+T 细胞在剧烈运动后 1 小时降至基线以下。
急性补充鱼油可减轻肥胖个体剧烈运动后的促炎细胞因子反应，并减少 DNA 损伤，表明其可能对肥胖个体剧烈运动引起的全身损伤恶化具有保护作用。
