McNatty K P, Lun S, Heath D A, O'Keeffe L E
J Reprod Fertil. 1987 Jan;79(1):57-66. doi: 10.1530/jrf.0.0790057.
The ovaries of 3-month-old Booroola lambs which were heterozygous carriers of a major gene (F) influencing the ovulation rate in mature ewes (i.e. F + lambs) were compared to those ofsimilarly-aged Booroola lambs which were non-carriers of the F-gene (i.e. ++ lambs). The ovaries of the F + Booroola lambs were significantly lighter (P less than 0.01) than those of ++ lambs even though the mean +/- s.e.m. number of follicles (greater than or equal to 1 mm diam.) in the F + lambs was greater than that in the ++ lambs (i.e. F + lambs, 30.2 +/- 2.5 follicles; ++ lambs, 18.4 +/- 1.2 follicles; P less than 0.01). In granulosa cells from non-atretic follicles (greater than or equal to 1 mm diam.) from F + and ++ Booroola lambs, FSH (NIAMDD-FSH-S16) doses of 100 and 1000 ng/ml caused significant stepwise increases (P less than 0.05) in cyclic adenosine 3',5'-monophosphate (cAMP) production compared to that achieved at FSH doses of 0 and 1 ng/ml or at any FSH dose in cells from atretic follicles. However, no significant differences in FSH-induced cAMP production were noted with regard to Booroola genotype or follicular diameter. None of the granulosa cell preparations from non-atretic follicles of 1-2.5 mm diameter from F + lambs (N = 13) or from non-atretic follicles of 1-4.5 mm diameter from ++ lambs (N = 16) responded to LH (NIAMDD-LH-S24; 10 or 1000 ng/ml) to produce significantly more cAMP than did the controls. In contrast, the granulosa cell preparations from non-atretic follicles of 3-4.5 mm diameter from F + lambs (N = 4) and from non-atretic follicles of greater than or equal to 5 mm diameter of ++ lambs (N = 4) produced significantly more cAMP (P less than 0.05) in response to LH (1000 and/or 10 ng/ml) relative to that in the controls. The theca interna from follicles of lambs of both genotypes had functional LH receptors as judged by the androstenedione responses to exogenous LH although no genotypic differences were noted. In F + lambs, the follicular fluid concentrations of testosterone but not oestradiol (i.e. in 1-4.5 mm diam. follicles) and granulosa cell aromatase activity (i.e. in 3-3.5 mm diam. follicles) were significantly higher (both P less than 0.05) than in corresponding follicles or cells from ++ lambs. Collectively the results suggest that the Booroola F-gene influences the composition and function of sheep ovaries before puberty.
将3月龄携带影响成年母羊排卵率的主基因（F）的布鲁拉羔羊（即F +羔羊）的卵巢，与同龄的不携带F基因的布鲁拉羔羊（即++羔羊）的卵巢进行比较。尽管F +羔羊中直径大于或等于1毫米的卵泡平均数量（±标准误）多于++羔羊（即F +羔羊为30.2±2.5个卵泡；++羔羊为18.4±1.2个卵泡；P<0.01），但F +布鲁拉羔羊的卵巢明显更轻（P<0.01）。在来自F +和++布鲁拉羔羊直径大于或等于1毫米的非闭锁卵泡的颗粒细胞中，与0和1纳克/毫升FSH剂量时或闭锁卵泡细胞中的任何FSH剂量相比，100和1000纳克/毫升的FSH（美国国立卫生研究院糖尿病、消化和肾脏疾病研究所 - FSH - S16）剂量导致环磷酸腺苷（cAMP）产量显著逐步增加（P<0.05）。然而，就布鲁拉基因型或卵泡直径而言，未观察到FSH诱导的cAMP产量有显著差异。来自F +羔羊直径1 - 2.5毫米的非闭锁卵泡（N = 13）或来自++羔羊直径1 - 4.5毫米的非闭锁卵泡的颗粒细胞制剂，对促黄体生成素（美国国立卫生研究院糖尿病、消化和肾脏疾病研究所 - LH - S24；10或1000纳克/毫升）均无反应，产生的cAMP量均未显著多于对照组。相比之下，来自F +羔羊直径3 - 4.5毫米的非闭锁卵泡（N = 4）和来自++羔羊直径大于或等于5毫米的非闭锁卵泡（N = 4）的颗粒细胞制剂，对促黄体生成素（1000和/或10纳克/毫升）的反应产生的cAMP量显著多于对照组（P<0.05）。根据对外源促黄体生成素的雄烯二酮反应判断，两种基因型羔羊卵泡的内膜具有功能性促黄体生成素受体，尽管未观察到基因型差异。在F +羔羊中，睾酮的卵泡液浓度显著高于++羔羊相应卵泡中的浓度（即直径1 - 4.5毫米的卵泡），但雌二醇浓度并非如此，且颗粒细胞芳香化酶活性（即直径3 - 3.5毫米的卵泡）也显著高于++羔羊相应细胞中的活性（两者P<0.05）。总体而言，结果表明布鲁拉F基因在青春期前影响绵羊卵巢的组成和功能。
