McNatty K P, Hudson N, Gibb M, Ball K, Henderson K M, Heath D A, Lun S, Kieboom L E
J Reprod Fertil. 1985 Sep;75(1):121-31. doi: 10.1530/jrf.0.0750121.
Injection of steroid-free bovine follicular fluid (bFF; 2 X 5 ml s.c. 12 h apart) into anoestrous ewes lowered plasma FSH concentrations by 70% and after 24 h had significantly (P less than 0.01) reduced the number of non-atretic follicles (greater than or equal to 1 mm diam.) without influencing the total number of follicles (greater than 1 mm diam.) compared to untreated controls. Hourly injections of FSH (10 micrograms i.v. NIH-FSH-S12) for 24 h did not influence the number of non-atretic follicles but did negate the inhibitory effects of bFF on follicular viability. Hourly injections of FSH (50 micrograms i.v., NIH-FSH-S12) + bFF treatment for 24 h significantly increased the total number of non-atretic follicles, and particularly the number of medium to large non-atretic follicles (greater than 3 mm diam.) compared to the untreated controls (both P less than 0.01). The 10 micrograms FSH regimen (without bFF) significantly increased aromatase activity in granulosa cells from large (greater than or equal to 5 mm diam.; P less than 0.01) but not medium (3-4.5 mm diam.) or small (1-2.5 mm diam.) follicles compared to controls. The 10 micrograms FSH + bFF regimen had no effect on granulosa-cell aromatase activity compared to the controls. However, the 50 micrograms FSH plus bFF regimen increased the aromatase activity of granulosa cells from large, medium and small non-atretic follicles 2.6-, 8.3- and greater than or equal to 11-fold respectively compared to that in the control cells. Ewes (N = 11) that ovulated 2 follicles had significantly higher plasma FSH concentrations from 48 to 24 h and 24 to 0 h before the onset of a cloprostenol-induced follicular phase (both P less than 0.01) than in the ewes (N = 12) that subsequently ovulated one follicle. Hourly FSH treatment (1.6 micrograms i.v., NIAMDD-FSH-S15) for 24 h but not for any 6 h intervals between 48 and 24 h or 24 and 0 h before a cloprostenol-induced luteolysis also resulted in significant increases (P less than 0.05) in the number of ewes with 2 ovulations.(ABSTRACT TRUNCATED AT 400 WORDS)
向处于乏情期的母羊皮下注射不含类固醇的牛卵泡液（bFF；2×5毫升，间隔12小时），可使血浆促卵泡素（FSH）浓度降低70%，24小时后，与未处理的对照组相比，非闭锁卵泡（直径大于或等于1毫米）数量显著减少（P<0.01），而卵泡总数（直径大于1毫米）不受影响。连续24小时每小时静脉注射促卵泡素（10微克，美国国立卫生研究院FSH-S12），对非闭锁卵泡数量没有影响，但消除了bFF对卵泡活力的抑制作用。连续24小时每小时静脉注射促卵泡素（50微克，美国国立卫生研究院FSH-S12）加bFF处理，与未处理的对照组相比，非闭锁卵泡总数显著增加，尤其是中到大的非闭锁卵泡（直径大于3毫米）数量增加（P均<0.01）。与对照组相比，10微克促卵泡素方案（无bFF）显著增加了大卵泡（直径大于或等于5毫米；P<0.01）颗粒细胞中的芳香化酶活性，但对中卵泡（3 - 4.5毫米）或小卵泡（1 - 2.5毫米）颗粒细胞中的芳香化酶活性无影响。与对照组相比，10微克促卵泡素加bFF方案对颗粒细胞芳香化酶活性无影响。然而，与对照细胞相比，50微克促卵泡素加bFF方案使大、中、小非闭锁卵泡颗粒细胞的芳香化酶活性分别增加了2.6倍、8.3倍和大于或等于11倍。在氯前列醇诱导的卵泡期开始前48至24小时和24至0小时，排卵2个卵泡的母羊（N = 11）血浆FSH浓度显著高于随后排卵1个卵泡的母羊（N = 12）（P均<0.01）。在氯前列醇诱导黄体溶解前连续24小时每小时静脉注射促卵泡素（1.6微克，美国国立医学研究与发展中心FSH-S15），而非在48至24小时或24至0小时之间的任何6小时间隔注射，也导致排卵2个卵泡的母羊数量显著增加（P<0.05）。（摘要截短至400字）
