Barry K, Stiles J I, Pietras D F, Melnick L, Sherman F
Mol Cell Biol. 1987 Feb;7(2):632-8. doi: 10.1128/mcb.7.2.632-638.1987.
Six genes, CYC1, UTR1, UTR3, OSM1, tRNAGly, and RAD7, have been localized within an 8-kilobase region on chromosome X of the yeast Saccharomyces cerevisiae. The physical structures and the transcripts of these genes were identified by analyzing a normal strain and six deletion mutants by genomic blotting, transcriptional analysis, and gene disruption procedures. The well-studied CYC1 gene encodes iso-1-cytochrome c; the tRNAGly gene encodes a tRNA; deletion of OSM1 and RAD7 causes sensitivity to hypertonic medium and UV irradiation, respectively. There were no observable phenotypes in strains having deletions of the UTR1, UTR3, and tRNAGly gene. The high density of transcripts, with little or almost no intragenic regions, indicates that the chromosomal organization of S. cerevisiae resembles the chromosomal organization of procaryotes rather than higher eucaryotes.
六个基因，CYC1、UTR1、UTR3、OSM1、tRNAGly和RAD7，已定位在酿酒酵母X染色体上一个8千碱基的区域内。通过基因组印迹、转录分析和基因破坏程序分析一个正常菌株和六个缺失突变体，确定了这些基因的物理结构和转录本。研究充分的CYC1基因编码异-1-细胞色素c；tRNAGly基因编码一种tRNA；缺失OSM1和RAD7分别导致对高渗培养基和紫外线照射敏感。UTR1、UTR3和tRNAGly基因缺失的菌株没有可观察到的表型。转录本密度高，基因内区域很少或几乎没有，这表明酿酒酵母的染色体组织类似于原核生物的染色体组织，而不是高等真核生物的。
