一个编码与人类c-has/bas原癌基因产物同源蛋白质的酵母基因。

A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene product.

作者信息

Gallwitz D, Donath C, Sander C

出版信息

Nature. 1983;306(5944):704-7. doi: 10.1038/306704a0.

Abstract

Organisms amenable to easy genetic analysis should prove helpful in assessing the function of at least those proto-oncogene products which are highly conserved in different eukaryotic cells. One obvious possibility is to pursue the matter in Drosophila melanogaster DNA, which has sequences homologous to several vertebrate oncogenes. Another is to turn to the yeast Saccharomyces cerevisiae, if it contains proto-oncogene sequences. Here we report the identification of a gene in S. cerevisiae which codes for a 206 amino acid protein (YP2) that exhibits striking homology to the p21 products of the human c-has/bas proto-oncogenes and the transforming p21 proteins of the Harvey (v-rasH) and Kirsten (v-rasK) murine sarcoma viral oncogenes. The YP2 gene is located between the actin and the tubulin gene on chromosome VI and is expressed in growing cells. The protein it encodes might share the nucleotide-binding capacity of p21 proteins.

摘要

适合进行简单遗传分析的生物体应该有助于评估至少那些在不同真核细胞中高度保守的原癌基因产物的功能。一种明显的可能性是在黑腹果蝇DNA中研究这个问题，它具有与几种脊椎动物癌基因同源的序列。另一种可能性是转向酿酒酵母，如果它含有原癌基因序列的话。在这里，我们报告在酿酒酵母中鉴定出一个基因，它编码一种206个氨基酸的蛋白质（YP2），该蛋白质与人类c-has/bas原癌基因的p21产物以及哈维（v-rasH）和柯尔斯滕（v-rasK）鼠肉瘤病毒癌基因的转化p21蛋白具有显著的同源性。YP2基因位于第六条染色体上的肌动蛋白基因和微管蛋白基因之间，并且在生长的细胞中表达。它编码的蛋白质可能具有与p21蛋白相同的核苷酸结合能力。