Department of Nephrology, The Second Affiliated Hospital, Army Medical University, Chongqing 400037, PR China; Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, PR China.
Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, PR China.
Clin Immunol. 2018 Dec;197:179-185. doi: 10.1016/j.clim.2018.10.001. Epub 2018 Oct 5.
Lupus nephritis (LN) is a common and devastating complication caused by systemic lupus erythematosus. In this study, we evaluated the expression and mechanism of Fos-related antigen 2 (Fra-2) in LN. The results showed that Fra-2 was significantly increased in kidney biopsies of LN patients compared with healthy controls and other kidney disease in glomerular podocytes. The MRL/lpr mouse strain is a murine model of lupus, and it was used to study the mechanisms of Fra-2 in LN. The results showed that Fra-2 was expressed in the glomerular podocytes. We investigated the effects of inflammatory stimuli on Fra-2 protein expression in the glomerular podocytes, and found that interferon gamma was most effective at increasing Fra-2 protein expression. Knockdown of Fra-2 using siRNA enhanced the protein expression of nephrin. Therefore, Fra-2 may be a specific drug target for podocyte injury in LN.
狼疮性肾炎 (LN) 是系统性红斑狼疮引起的一种常见且严重的并发症。在本研究中，我们评估了 Fos 相关抗原 2 (Fra-2) 在 LN 中的表达和机制。结果表明，与健康对照组和其他肾小球疾病相比，LN 患者的肾活检中 Fra-2 显著增加。MRL/lpr 小鼠品系是狼疮的一种小鼠模型，用于研究 Fra-2 在 LN 中的作用机制。结果表明，Fra-2 在肾小球足细胞中表达。我们研究了炎性刺激对肾小球足细胞 Fra-2 蛋白表达的影响，发现干扰素 γ 最有效地增加 Fra-2 蛋白表达。用 siRNA 敲低 Fra-2 可增强nephrin 的蛋白表达。因此，Fra-2 可能是 LN 中足细胞损伤的特定药物靶点。
