Lo Leggio Leila, Weihe Cecilia D, Poulsen Jens-Christian N, Sweeney Matt, Rasmussen Frank, Lin Janine, De Maria Leonardo, Wogulis Mark
Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
Department of Chemistry, University of Copenhagen, Universitetsparken 5, 2100, Copenhagen, Denmark.
Carbohydr Res. 2018 Nov;469:55-59. doi: 10.1016/j.carres.2018.08.009. Epub 2018 Aug 14.
Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.
裂解多糖单加氧酶（LPMOs）是一类工业酶，通过与糖苷水解酶协同作用，在从木质纤维素生产第二代生物乙醇中得到越来越广泛的应用。在此，我们展示了烟曲霉AA9真菌LPMO及其一个变体的X射线晶体结构，该变体已被证明在高温下具有更好的性能。基于这些结构、热变性数据和理论计算，我们对稳定性提高的结构基础提出了建议。
