插入到SP6聚合酶转录载体起始位点下游两个碱基处的DNA在体外能被高效转录。 - Suppr

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超能文献

DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.

作者信息

Bujarski J J, Kaesberg P

出版信息

Nucleic Acids Res. 1987 Feb 11;15(3):1337. doi: 10.1093/nar/15.3.1337.

DOI:10.1093/nar/15.3.1337

PMID:3029707

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340536/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2bf/340536/09aca9206c32/nar00247-0464-a.jpg

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DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.插入到SP6聚合酶转录载体起始位点下游两个碱基处的DNA在体外能被高效转录。

Nucleic Acids Res. 1987 Feb 11;15(3):1337. doi: 10.1093/nar/15.3.1337.

Studies on SP6 promoter using a new plasmid vector that allows gene insertion at the transcription initiation site.使用一种新型质粒载体对SP6启动子进行研究，该载体允许在转录起始位点插入基因。

Nucleic Acids Res. 1987 Mar 11;15(5):2279-94. doi: 10.1093/nar/15.5.2279.

Suitability of SP6 RNA polymerase transcripts for in vitro viral diagnosis.SP6 RNA聚合酶转录本用于体外病毒诊断的适用性。

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Transcription initiation site selection and abortive initiation cycling of phage SP6 RNA polymerase.噬菌体SP6 RNA聚合酶的转录起始位点选择与流产起始循环

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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。

Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.

SP6 RNA polymerase stutters when initiating from an AAA... sequence.SP6 RNA聚合酶从AAA...序列起始时会发生口吃现象。

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Initiation of plasmid R1 replication in vitro is independent of transcription by host RNA polymerase.质粒R1在体外的复制起始不依赖于宿主RNA聚合酶的转录。

Nucleic Acids Res. 1984 Jul 11;12(13):5175-91. doi: 10.1093/nar/12.13.5175.

SP6 RNA polymerase efficiently synthesizes RNA from short double-stranded DNA templates.SP6 RNA聚合酶能有效地从短双链DNA模板合成RNA。

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pTRIPLEscript: a novel cloning vector for generating in vitro transcripts from tandem promoters for SP6, T7 and T3 RNA polymerase.

Biotechniques. 1995 Jan;18(1):152-7,.

In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.不含5'载体核苷酸的嵌合SP6信使核糖核酸的体外转录和翻译效率

Nucleic Acids Res. 1988 May 25;16(10):4483-98. doi: 10.1093/nar/16.10.4483.

引用本文的文献

The molecular variability analysis of the RNA 3 of fifteen isolates of Prunus necrotic ringspot virus sheds light on the minimal requirements for the synthesis of its subgenomic RNA.对十五株李坏死环斑病毒的RNA 3进行分子变异性分析，揭示了其亚基因组RNA合成的最低要求。

Virus Genes. 2002;25(1):75-84. doi: 10.1023/a:1020126309692.

In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.不含5'载体核苷酸的嵌合SP6信使核糖核酸的体外转录和翻译效率

Nucleic Acids Res. 1988 May 25;16(10):4483-98. doi: 10.1093/nar/16.10.4483.

A plasmid vector for cloning directly at the transcription initiation site of a bacteriophage T7 promoter.一种用于直接在噬菌体T7启动子转录起始位点进行克隆的质粒载体。

Nucleic Acids Res. 1988 Sep 12;16(17):8738. doi: 10.1093/nar/16.17.8738.

Derivation of an infectious viral RNA by autolytic cleavage of in vitro transcribed viral cDNAs.通过体外转录的病毒cDNA的自溶切割产生感染性病毒RNA。

Proc Natl Acad Sci U S A. 1989 Jul;86(13):4823-7. doi: 10.1073/pnas.86.13.4823.

An in vitro transcription vector which generates nearly correctly ended RNAs by self-cleavage of longer transcripts.一种体外转录载体，可通过较长转录本的自我切割产生近乎正确末端的RNA。

Nucleic Acids Res. 1988 Nov 25;16(22):10940. doi: 10.1093/nar/16.22.10940.

本文引用的文献

Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication.雀麦花叶病毒基因组的核苷酸序列及其对病毒复制的影响。

J Mol Biol. 1984 Feb 5;172(4):369-83. doi: 10.1016/s0022-2836(84)80012-1.

Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.

Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.通过从昆虫病毒基因组RNA的克隆cDNA拷贝转录而来的感染性RNA。

Proc Natl Acad Sci U S A. 1986 Jan;83(1):63-6. doi: 10.1073/pnas.83.1.63.

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