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DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.

作者信息

Bujarski J J, Kaesberg P

出版信息

Nucleic Acids Res. 1987 Feb 11;15(3):1337. doi: 10.1093/nar/15.3.1337.

DOI:10.1093/nar/15.3.1337
PMID:3029707
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340536/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2bf/340536/09aca9206c32/nar00247-0464-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2bf/340536/09aca9206c32/nar00247-0464-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a2bf/340536/09aca9206c32/nar00247-0464-a.jpg

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DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.插入到SP6聚合酶转录载体起始位点下游两个碱基处的DNA在体外能被高效转录。
Nucleic Acids Res. 1987 Feb 11;15(3):1337. doi: 10.1093/nar/15.3.1337.
2
Studies on SP6 promoter using a new plasmid vector that allows gene insertion at the transcription initiation site.使用一种新型质粒载体对SP6启动子进行研究，该载体允许在转录起始位点插入基因。
Nucleic Acids Res. 1987 Mar 11;15(5):2279-94. doi: 10.1093/nar/15.5.2279.
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Suitability of SP6 RNA polymerase transcripts for in vitro viral diagnosis.SP6 RNA聚合酶转录本用于体外病毒诊断的适用性。
Clin Chem. 1985 Dec;31(12):2043-4.
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Transcription initiation site selection and abortive initiation cycling of phage SP6 RNA polymerase.噬菌体SP6 RNA聚合酶的转录起始位点选择与流产起始循环
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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
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SP6 RNA polymerase stutters when initiating from an AAA... sequence.SP6 RNA聚合酶从AAA...序列起始时会发生口吃现象。
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Initiation of plasmid R1 replication in vitro is independent of transcription by host RNA polymerase.质粒R1在体外的复制起始不依赖于宿主RNA聚合酶的转录。
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SP6 RNA polymerase efficiently synthesizes RNA from short double-stranded DNA templates.SP6 RNA聚合酶能有效地从短双链DNA模板合成RNA。
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pTRIPLEscript: a novel cloning vector for generating in vitro transcripts from tandem promoters for SP6, T7 and T3 RNA polymerase.
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In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.不含5'载体核苷酸的嵌合SP6信使核糖核酸的体外转录和翻译效率
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The molecular variability analysis of the RNA 3 of fifteen isolates of Prunus necrotic ringspot virus sheds light on the minimal requirements for the synthesis of its subgenomic RNA.对十五株李坏死环斑病毒的RNA 3进行分子变异性分析，揭示了其亚基因组RNA合成的最低要求。
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In vitro transcription and translational efficiency of chimeric SP6 messenger RNAs devoid of 5' vector nucleotides.不含5'载体核苷酸的嵌合SP6信使核糖核酸的体外转录和翻译效率
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A plasmid vector for cloning directly at the transcription initiation site of a bacteriophage T7 promoter.

本文引用的文献

1
Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication.雀麦花叶病毒基因组的核苷酸序列及其对病毒复制的影响。
J Mol Biol. 1984 Feb 5;172(4):369-83. doi: 10.1016/s0022-2836(84)80012-1.
2
Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.从含有噬菌体SP6启动子的质粒中高效体外合成生物活性RNA和RNA杂交探针。
Nucleic Acids Res. 1984 Sep 25;12(18):7035-56. doi: 10.1093/nar/12.18.7035.
3
Infectious RNA derived by transcription from cloned cDNA copies of the genomic RNA of an insect virus.
一种用于直接在噬菌体T7启动子转录起始位点进行克隆的质粒载体。
Nucleic Acids Res. 1988 Sep 12;16(17):8738. doi: 10.1093/nar/16.17.8738.
4
Derivation of an infectious viral RNA by autolytic cleavage of in vitro transcribed viral cDNAs.通过体外转录的病毒cDNA的自溶切割产生感染性病毒RNA。
Proc Natl Acad Sci U S A. 1989 Jul;86(13):4823-7. doi: 10.1073/pnas.86.13.4823.
5
An in vitro transcription vector which generates nearly correctly ended RNAs by self-cleavage of longer transcripts.一种体外转录载体，可通过较长转录本的自我切割产生近乎正确末端的RNA。
Nucleic Acids Res. 1988 Nov 25;16(22):10940. doi: 10.1093/nar/16.22.10940.
通过从昆虫病毒基因组RNA的克隆cDNA拷贝转录而来的感染性RNA。
Proc Natl Acad Sci U S A. 1986 Jan;83(1):63-6. doi: 10.1073/pnas.83.1.63.