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A plasmid vector for cloning directly at the transcription initiation site of a bacteriophage T7 promoter.一种用于直接在噬菌体T7启动子转录起始位点进行克隆的质粒载体。
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2
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Genome mapping and protein coding region identification using bacteriophage Mu.利用噬菌体Mu进行基因组图谱绘制和蛋白质编码区鉴定。
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[Cloning and study of the structural organization of regions of the inh(lip)-hoc genes of bacteriophage T4].[噬菌体T4 inh(lip)-hoc基因区域的结构组织克隆与研究]
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Bacteriophage T7 late promoters with point mutations: quantitative footprinting and in vivo expression.具有点突变的噬菌体T7晚期启动子:定量足迹法与体内表达
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Phage T4 expression vector: protection from proteolysis.
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J Mol Biol. 1988 Dec 20;204(4):903-16. doi: 10.1016/0022-2836(88)90050-2.
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Creation of a T7 autogene. Cloning and expression of the gene for bacteriophage T7 RNA polymerase under control of its cognate promoter.构建T7自基因。在其同源启动子控制下克隆和表达噬菌体T7 RNA聚合酶基因。
J Mol Biol. 1991 May 5;219(1):61-8. doi: 10.1016/0022-2836(91)90857-3.

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The RNA of Maize Chlorotic Mottle Virus, an Obligatory Component of Maize Lethal Necrosis Disease, Is Translated via a Variant Panicum Mosaic Virus-Like Cap-Independent Translation Element.玉米线条病毒 RNA,玉米坏死性条纹病的必需成分,通过类似百慕大 mosaic 病毒的帽非依赖性翻译元件进行翻译。
J Virol. 2020 Oct 27;94(22). doi: 10.1128/JVI.01005-20.
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Intron insertion facilitates amplification of cloned virus cDNA in Escherichia coli while biological activity is reestablished after transcription in vivo.内含子插入有助于在大肠杆菌中扩增克隆的病毒cDNA,而在体内转录后可重新建立生物学活性。
Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12400-5. doi: 10.1073/pnas.93.22.12400.
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In vivo restoration of biologically active 3' ends of virus-associated RNAs by nonhomologous RNA recombination and replacement of a terminal motif.通过非同源RNA重组和末端基序替换在体内恢复病毒相关RNA的生物活性3'末端
J Virol. 1996 Jan;70(1):478-86. doi: 10.1128/JVI.70.1.478-486.1996.
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The middle hepatitis B virus envelope protein is not necessary for infectivity of hepatitis delta virus.乙型肝炎病毒中间包膜蛋白对于丁型肝炎病毒的感染性并非必需。
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RNA-dependent RNA polymerase from plants infected with turnip crinkle virus can transcribe (+)- and (-)-strands of virus-associated RNAs.感染芜菁皱缩病毒的植物中的RNA依赖性RNA聚合酶能够转录病毒相关RNA的正链和负链。
Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8792-6. doi: 10.1073/pnas.91.19.8792.
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BSMV genome mediated expression of a foreign gene in dicot and monocot plant cells.
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7
In vivo accumulation of a turnip crinkle virus defective interfering RNA is affected by alterations in size and sequence.芜菁皱缩病毒缺陷干扰RNA的体内积累受大小和序列改变的影响。
J Virol. 1991 Sep;65(9):4582-90. doi: 10.1128/JVI.65.9.4582-4590.1991.
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Single amino acid change in the helicase domain of the putative RNA replicase of turnip crinkle virus alters symptom intensification by virulent satellites.芜菁皱缩病毒假定RNA复制酶解旋酶结构域中的单氨基酸变化改变了强毒性卫星病毒引起的症状加重情况。
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):309-13. doi: 10.1073/pnas.89.1.309.

本文引用的文献

1
Production of single-stranded plasmid DNA.单链质粒DNA的制备。
Methods Enzymol. 1987;153:3-11. doi: 10.1016/0076-6879(87)53044-0.
2
Studies on SP6 promoter using a new plasmid vector that allows gene insertion at the transcription initiation site.使用一种新型质粒载体对SP6启动子进行研究,该载体允许在转录起始位点插入基因。
Nucleic Acids Res. 1987 Mar 11;15(5):2279-94. doi: 10.1093/nar/15.5.2279.
3
DNA inserted two bases down from the initiation site of a SP6 polymerase transcription vector is transcribed efficiently in vitro.插入到SP6聚合酶转录载体起始位点下游两个碱基处的DNA在体外能被高效转录。
Nucleic Acids Res. 1987 Feb 11;15(3):1337. doi: 10.1093/nar/15.3.1337.

A plasmid vector for cloning directly at the transcription initiation site of a bacteriophage T7 promoter.

作者信息

Petty I T

机构信息

Department of Plant Pathology, University of California, Berkeley 94720.

出版信息

Nucleic Acids Res. 1988 Sep 12;16(17):8738. doi: 10.1093/nar/16.17.8738.

DOI:10.1093/nar/16.17.8738
PMID:3047690
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC338616/
Abstract
摘要