Gudz' T I, Gorodovikova E N, Boushev V G, Pandelova I G, Goncharenko E N
Radiobiologiia. 1987 Jan-Feb;27(1):115-8.
A study was made of generation of superoxide anion-radical (O2-) by cytochrome P-450 in a microsomal membrane of rat liver. Using spectrophotometry (by oxidation of adrenaline to adrenochrome) and ESR (with a spin-trap, tiron) the authors showed the ability of O2- generation by P-450 through decomposition of organic peroxides. During the first 24 h following irradiation of rats with doses of 7 and 10 Gy, the generation of O2- by cytochrome P-450 of rat liver microsomes was increased. Mechanisms of the postirradiation modification of O2- generation rate are discussed.
对大鼠肝脏微粒体膜中细胞色素P-450产生超氧阴离子自由基（O2-）进行了研究。作者利用分光光度法（通过将肾上腺素氧化为肾上腺色素）和电子自旋共振（使用自旋捕获剂钛铁试剂）表明，P-450可通过有机过氧化物的分解产生O2-。在用7和10 Gy剂量照射大鼠后的最初24小时内，大鼠肝脏微粒体细胞色素P-450产生O2-的量增加。讨论了照射后O2-产生速率改变的机制。
