Fauzia Eram, Barbhuyan Tarun Kumar, Shrivastava Amit Kumar, Kumar Manish, Garg Paarth, Khan Mohsin Ali, Robertson Avril A B, Raza Syed Shadab
Laboratory for Stem Cell and Restorative Neurology, Department of Biotechnology, Era's Lucknow Medical College and Hospital, Era University, Lucknow, India.
Era's Lucknow Medical College and Hospital, Era University, Lucknow, India.
Front Pharmacol. 2018 Sep 21;9:1034. doi: 10.3389/fphar.2018.01034. eCollection 2018.
Ischemia-reperfusion (I/R)-related disorders, such as stroke, myocardial infarction, and peripheral vascular disease, are among the most frequent causes of disease and death. Tissue injury or death may result from the initial ischemic insult, primarily determined by the magnitude and duration of the interruption in blood supply and then by the subsequent reperfusion-induced damage. Various and models are currently available to study I/R mechanism in the brain and other tissues. However, thus far, no I/R model has been reported for understanding the I/R mechanisms and for faster drug screening. Here, we developed an Hook model of I/R by occluding and releasing the right vitelline artery of a chick embryo at 72 h of development. To validate the model and elucidate various underlying survival and death mechanisms, we employed imaging (Doppler blood flow imaging), biochemical, and blotting techniques and evaluated the cell death mechanism: autophagy and inflammation caused by I/R. In conclusion, the present model is useful in parallel with established and I/R models to understand the mechanisms of I/R development and its treatment.
缺血再灌注（I/R）相关疾病，如中风、心肌梗死和外周血管疾病，是最常见的疾病和死亡原因之一。组织损伤或死亡可能源于最初的缺血性损伤，主要取决于血液供应中断的程度和持续时间，随后还取决于再灌注诱导的损伤。目前有各种模型可用于研究大脑和其他组织中的I/R机制。然而，迄今为止，尚未有用于理解I/R机制和更快进行药物筛选的I/R模型报道。在此，我们通过在发育72小时的鸡胚中阻断并释放右卵黄动脉，建立了一种I/R的鸡胚模型。为了验证该模型并阐明各种潜在的存活和死亡机制，我们采用了成像（多普勒血流成像）、生化和印迹技术，并评估了细胞死亡机制：I/R引起的自噬和炎症。总之，本模型与已建立的I/R模型并行，有助于理解I/R发展及其治疗的机制。
