Khandia Rekha, Pathe Chandra Shekhar, Vishwakarma Pratibha, Dhama Kuldeep, Munjal Ashok
Department of Biochemistry and Genetics, Barkatullah University, Bhopal, 462026, Madhya Pradesh, India.
Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, 243 122, Uttar Pradesh, India.
J Ayurveda Integr Med. 2020 Oct-Dec;11(4):495-501. doi: 10.1016/j.jaim.2019.03.003. Epub 2019 Nov 19.
Insecticides are widely used in agriculture to curb the loss caused by insects. These insecticides are incorporated into the food chain and accumulate in the human body, as well disturb the various metabolic pathways. Imidacloprid is an insect neurotoxin commonly used in agriculture to control the insect pests. P. niruri is a traditional medicinal shrub widely used as an anti-inflammatory, antipyretic, and anti-lethality agent.
The present study is designed to evaluate the ameliorative effects of Phyllanthus niruri (Bhumi amla) on the deleterious Insecticide imidacloprid in the vital organs of Chicken embryos.
The embryonated chicken eggs were divided into the four groups (one control and three treated groups); the chorioallantoic membranes of control received 200 μl phosphate buffer saline, whereas group I and group II received 100 μg imidacloprid and 200 μl aqueous extract of P. niruri (PNE) respectively. Group III received both 100 μg imidacloprid and 200 μl PNE. The serum was collected on the 18th day its development; which was subjected to the biochemical analysis based on colorimetric assay in semi-automated biochemical analyzer using commercial kits.
We observed significant in ovo effects of imidacloprid on chicken embryos; the values of aspartate aminotransferase (AST), alanine aminotransferase (ALT), were increased in imidacloprid treated group I; histopathology also revealed damage to the liver (necrotic areas and dilated blood sinusoids). Alkaline phosphatase (ALP), amylase, cholesterol, triglycerides protein and albumin levels were also altered significantly (p < 0.05).
The serum biochemicals were returned back to the nearly normal levels. PNE has ameliorated and overcome the effects of imidacloprid reasonably with the subsequent treatment among group III. Hence, P. niruri may be used to minimize the effects of an accidental exposure of imidacloprid.
杀虫剂在农业中被广泛用于减少昆虫造成的损失。这些杀虫剂进入食物链并在人体中积累，还会干扰各种代谢途径。吡虫啉是一种常用于农业防治害虫的昆虫神经毒素。叶下珠是一种传统药用灌木，广泛用作抗炎、解热和抗致死剂。
本研究旨在评估叶下珠对鸡胚重要器官中有害杀虫剂吡虫啉的改善作用。
将受精鸡蛋分为四组（一组对照，三组处理组）；对照组的尿囊膜接受200μl磷酸盐缓冲盐水，而第一组和第二组分别接受100μg吡虫啉和200μl叶下珠水提取物（PNE）。第三组同时接受100μg吡虫啉和200μl PNE。在其发育的第18天收集血清；使用商业试剂盒在半自动生化分析仪中基于比色法对其进行生化分析。
我们观察到吡虫啉对鸡胚有显著的体内效应；在吡虫啉处理的第一组中，天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）的值升高；组织病理学也显示肝脏受损（坏死区域和扩张的血窦）。碱性磷酸酶（ALP）、淀粉酶、胆固醇、甘油三酯、蛋白质和白蛋白水平也有显著改变（p<0.05）。
血清生化指标恢复到接近正常水平。PNE在第三组的后续处理中合理地改善并克服了吡虫啉的影响。因此，叶下珠可用于尽量减少意外接触吡虫啉的影响。
