前列腺素对胃黏液糖蛋白物理性质及其对胃蛋白酶敏感性的影响。

Prostaglandin effect on the physical properties of gastric mucus glycoprotein and its susceptibility to pepsin.

作者信息

Sarosiek J, Murty V L, Nadziejko C, Slomiany A, Slomiany B L

出版信息

Prostaglandins. 1986 Nov;32(5):635-46. doi: 10.1016/0090-6980(86)90186-3.

Abstract

The effect of 16,16-dimethyl prostaglandin E2 (DMPGE2) on gastric mucus glycoprotein viscosity, permeability to hydrogen ion and degradation by pepsin was investigated. Preincubation with DMPGE2 produced a marked enhancement in the glycoprotein viscosity. The increase was concentration dependent and at 2.6 X 10(-5)M DMPGE2 reached a value of 178%. Permeability measurements revealed that 2.6 X 10(-7)M DMPGE2 increased the retardation ability of the glycoprotein to hydrogen ion by 10%, while 22% increase was obtained with 2.6 X 10(-4)M DMPGE2. The results of peptic activity assay showed that DMPGE2 had no inhibitory effect on the rate of glycoprotein proteolysis, and actually a small stimulatory influence was consistently observed. The results suggest that prostaglandins beneficially affect the physical properties of mucus glycoprotein which are considered to be essential for the protective function of gastric mucus.

摘要

研究了16,16 - 二甲基前列腺素E2（DMPGE2）对胃黏液糖蛋白黏度、氢离子通透性及胃蛋白酶降解作用的影响。用DMPGE2预孵育可使糖蛋白黏度显著增强。这种增加呈浓度依赖性，在2.6×10（-5）M DMPGE2时达到178%的值。通透性测量显示，2.6×10（-7）M DMPGE2使糖蛋白对氢离子的阻滞能力增加10%，而2.6×10（-4）M DMPGE2则使阻滞能力增加22%。胃蛋白酶活性测定结果表明，DMPGE2对糖蛋白的蛋白水解速率没有抑制作用，实际上还一直观察到有轻微的刺激作用。结果提示，前列腺素对黏液糖蛋白的物理性质有有益影响，而这些物理性质被认为对胃黏液的保护功能至关重要。