Department of Pharmacology, Medical College, Yangzhou University, Yangzhou, China.
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases, Yangzhou University, Yangzhou, China.
J Pharm Pharmacol. 2019 Feb;71(2):220-229. doi: 10.1111/jphp.13023. Epub 2018 Oct 9.
The objective of this paper was to explore the effects of Radix isatidis polysaccharide (RIP) extracted from Radix isatis on alleviating insulin resistance.
The insulin resistance models of 3T3-L1 preadipocytes and type 2 diabetic rats were established to evaluate the insulin resistance activity of RIP.
Radix isatidis polysaccharide within the concentration range of 25-100 μg/ml could reduce cell supernatant glucose and TNF-α levels (P < 0.01) and increase the expression of PI-3K P85, Glut4, IRS-1 and Akt protein in symptoms of IR 3T3-L1 preadipocytes. In the meantime, RIP contributed to relieve the weight loss of diabetic rats whose liver weight and liver index were decreased due to the effects of RIP. Experiments in rats also showed that RIP had capacity in reduced serum TC, TG, LDL-C, FFA, FBG, FINS, MDA, ALT, AST activities and increased serum HDL-C, SOD, ISI (P < 0.05 or 0.01). In addition, the oral glucose tolerance in rats was improved (P < 0.05) and liver damage was restored due to RIP.
Radix isatidis polysaccharide significantly alleviates insulin resistance in 3T3-L1 preadipocytes and type 2 diabetic rats. These beneficial effects of RIP may associate with their roles in improving the glucose metabolism, lipid metabolism and oxidative stress.
本文旨在探讨板蓝根多糖（RIP）对改善胰岛素抵抗的作用。
建立 3T3-L1 前脂肪细胞和 2 型糖尿病大鼠胰岛素抵抗模型，评价 RIP 的胰岛素抵抗活性。
在 25-100μg/ml 的浓度范围内，板蓝根多糖可降低细胞上清液葡萄糖和 TNF-α水平（P<0.01），并增加胰岛素抵抗 3T3-L1 前脂肪细胞中 PI-3K P85、Glut4、IRS-1 和 Akt 蛋白的表达。同时，RIP 有助于减轻因 RIP 作用而导致的糖尿病大鼠体重减轻，降低其肝重和肝指数。大鼠实验还表明，RIP 具有降低血清 TC、TG、LDL-C、FFA、FBG、FINS、MDA、ALT、AST 活性和增加血清 HDL-C、SOD、ISI 的能力（P<0.05 或 0.01）。此外，RIP 还改善了大鼠的口服糖耐量（P<0.05），并修复了肝损伤。
板蓝根多糖可显著改善 3T3-L1 前脂肪细胞和 2 型糖尿病大鼠的胰岛素抵抗。RIP 的这些有益作用可能与其改善葡萄糖代谢、脂代谢和氧化应激的作用有关。
