Han Yo-Han, Kee Ji-Ye
Department of Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Department of Oriental Pharmacy, College of Pharmacy, Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan 54538, Republic of Korea.
Antioxidants (Basel). 2023 Jul 14;12(7):1426. doi: 10.3390/antiox12071426.
(IR), the root of L. belonging to Brassicaceae, has been traditionally used as a fever reducer. Although some pharmacological effects, such as anti-diabetes, anti-virus, and anti-inflammatory, have been reported, there is no study on the anti-obesity effect of IR. This study used 3T3-L1 cells, human mesenchymal adipose stem cells (hAMSCs), and a high-fat diet (HFD)-induced obese mouse model to confirm the anti-adipogenic effect of IR. Intracellular lipid accumulation in 3T3-L1 cells and hAMSCs was decreased by IR treatment.IR extract especially suppressed reactive oxygen species (ROS) production through a cluster of differentiation 36 (CD36)-AMP-activated protein kinase (AMPK) pathway. Consequently, the expressions of peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT-enhancer-binding proteins alpha (C/EBPα), and fatty acid synthesis (FAS) were inhibited by IR extract. In addition, β-oxidation-related genes were also decreased by treatment of IR extract. IR inhibited weight gain through this cascade in the HFD-induced obese mouse model. IR significantly suppressed lipid accumulation in epididymal white adipose tissue (eWAT). Furthermore, the administration of IR extract decreased serum free fatty acid (FFA), total cholesterol (TC), and LDL cholesterol, suggesting that it could be a potential drug for obesity by inhibiting lipid accumulation.
十字花科植物萝卜（Raphanus sativus L.）的根，传统上被用作退烧药。尽管已经报道了一些药理作用，如抗糖尿病、抗病毒和抗炎作用，但尚未有关于萝卜根抗肥胖作用的研究。本研究使用3T3-L1细胞、人间充质脂肪干细胞（hAMSCs）和高脂饮食（HFD）诱导的肥胖小鼠模型来证实萝卜根的抗脂肪生成作用。萝卜根处理可减少3T3-L1细胞和hAMSCs中的细胞内脂质积累。萝卜根提取物尤其通过分化簇36（CD36）-AMP激活蛋白激酶（AMPK）途径抑制活性氧（ROS）的产生。因此，萝卜根提取物抑制了过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT增强子结合蛋白α（C/EBPα）和脂肪酸合成（FAS）的表达。此外，萝卜根提取物处理还降低了β-氧化相关基因的表达。在HFD诱导的肥胖小鼠模型中，萝卜根通过这一系列作用抑制体重增加。萝卜根显著抑制附睾白色脂肪组织（eWAT）中的脂质积累。此外，萝卜根提取物的给药降低了血清游离脂肪酸（FFA）、总胆固醇（TC）和低密度脂蛋白胆固醇，表明它可能是一种通过抑制脂质积累来治疗肥胖的潜在药物。
