Caren L D, Brunmeier V
Toxicol Lett. 1987 Feb;35(2-3):277-84. doi: 10.1016/0378-4274(87)90217-7.
In a double-blind study, mice were injected intraperitoneally with 0.2 ml 0.2% octoxynol-9 (O-9), 0.2 ml 0.2% nonoxynol-9 (N-9), or 0.2 ml saline (control) daily for 24 days. Another control group received no treatment. All mice were immunized twice with sheep red blood cells (SRBC) and bled by caudal incision. Mice receiving N-9 lost weight (P less than 0.02), had smaller livers (P less than 0.05), and showed enlarged spleens (P less than 0.05). The N-9-treated mice did not differ from either control group in the primary or secondary anti-SRBC responses, leukocyte (WBC) counts, or in the sizes of the kidneys, hearts, lungs, or thymuses. Mice receiving O-9 showed no significant differences from either control group in any of these tests. Serum immunoglobulin M (IgM) and immunoglobulin G (IgG) levels were similar in mice treated with O-9, N-9, or saline. All 3 groups had higher levels of both classes of immunoglobulins on day 16 than did untreated controls. This study shows that O-9, given to mice in doses 3 times that used by humans, is nontoxic, whereas the same dose of N-9 has minor deleterious effects.
在一项双盲研究中，小鼠每天腹腔注射0.2毫升0.2%的辛苯聚醇-9（O-9）、0.2毫升0.2%的壬苯聚醇-9（N-9）或0.2毫升生理盐水（对照组），持续24天。另一个对照组不接受任何治疗。所有小鼠均用绵羊红细胞（SRBC）免疫两次，并通过尾静脉切开取血。接受N-9的小鼠体重减轻（P<0.02），肝脏较小（P<0.05），脾脏肿大（P<0.05）。接受N-9治疗的小鼠在初次或二次抗SRBC反应、白细胞（WBC）计数以及肾脏、心脏、肺或胸腺大小方面与两个对照组均无差异。接受O-9的小鼠在任何这些测试中与两个对照组均无显著差异。用O-9、N-9或生理盐水处理的小鼠血清免疫球蛋白M（IgM）和免疫球蛋白G（IgG）水平相似。所有三组在第16天时这两类免疫球蛋白的水平均高于未处理的对照组。这项研究表明，以人类使用剂量的3倍给予小鼠的O-9是无毒的，而相同剂量的N-9有轻微的有害作用。
