Caren L D, Oven H M, Mandel A D
Toxicol Lett. 1985 Aug;26(2-3):193-7. doi: 10.1016/0378-4274(85)90166-3.
Mice were injected intraperitoneally (i.p.) with 100% dimethyl sulfoxide (DMSO) or sterile saline (controls) for 36 days. The mice received 0.05 ml daily for one week, 0.025 ml every other day for the second week (because the DMSO-treated mice appeared weak), and 0.05 ml daily for 3 more weeks. All mice were immunized twice with sheep red blood cells (days 13 and 24), and bled twice by caudal incision (days 20 and 29). Hematocrits were significantly decreased (P less than or equal to 0.002) but still within the normal range. The primary and secondary antibody response to sheep red blood cells (SRBC), leukocyte counts, body weight, and the size of the heart, lungs, spleen, thymus, and kidneys were not affected. DMSO treatment resulted in significant liver enlargement (P = 0.02). It is concluded that this dose of DMSO is not deleterious to the humoral immune response in mice responding to a new antigen.
给小鼠腹腔注射（i.p.）100%二甲基亚砜（DMSO）或无菌生理盐水（对照组），持续36天。小鼠第一周每天接受0.05毫升注射，第二周每隔一天接受0.025毫升注射（因为用DMSO处理的小鼠显得虚弱），之后3周每天接受0.05毫升注射。所有小鼠用绵羊红细胞免疫两次（第13天和第24天），并通过尾静脉切开术采血两次（第20天和第29天）。血细胞比容显著降低（P≤0.002），但仍在正常范围内。对绵羊红细胞（SRBC）的初次和二次抗体反应、白细胞计数、体重以及心脏、肺、脾脏、胸腺和肾脏的大小均未受影响。DMSO处理导致肝脏显著肿大（P = 0.02）。得出的结论是，该剂量的DMSO对响应新抗原的小鼠体液免疫反应无害。
