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口疮病毒DNA的结构与克隆

The structure and cloning of orf virus DNA.

作者信息

Mercer A A, Fraser K, Barns G, Robinson A J

出版信息

Virology. 1987 Mar;157(1):1-12. doi: 10.1016/0042-6822(87)90307-2.

DOI:10.1016/0042-6822(87)90307-2
PMID:3029950
Abstract

A map of cleavage sites for the restriction endonucleases EcoRI, HindIII, BamHI, HpaI, and KpnI for a New Zealand strain of orf virus (NZ2) DNA has been deduced. Also, the entire genome, apart from approximately 0.1 kbp at each end, has been cloned into various vectors. The genome is 139 kbp in length and, in common with other poxviruses, has inverted terminal repetitions and crosslinked ends.

摘要

已推导出去氧核糖核酸酶EcoRI、HindIII、BamHI、HpaI和KpnI对新西兰株羊口疮病毒(NZ2)DNA的切割位点图谱。此外,除两端各约0.1千碱基对(kbp)外,整个基因组已被克隆到各种载体中。该基因组长度为139 kbp,与其他痘病毒一样,具有反向末端重复序列和交联末端。

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