Mercer J A, Marks J R, Spector D H
Virology. 1983 Aug;129(1):94-106. doi: 10.1016/0042-6822(83)90398-7.
We have cloned EcoRI and HindIII fragments of the Smith strain of murine cytomegalovirus (MCMV) in the plasmid vector pACYC184. These cloned fragments were used to establish a restriction endonuclease map of the genome with respect to the EcoRI and HindIII sites. The map was constructed on the basis of data derived from cross-hybridizations of EcoRI and HindIII cloned fragments, double-digestions of the cloned fragments with EcoRI and HindIII, and hybridization of cloned HindIII fragments to Southern blots of MCMV DNA cleaved with EcoRI. From our mapping data, we have determined that the length of the MCMV genome is approximately 240 kbp. The genome does not appear to undergo inversions and lacks detectable repeated sequences. One HindIII cloned fragment was obtained which contained both HindIII termini. The existence of this fragment may be related to the mode of replication of the MCMV genome.
我们已将鼠巨细胞病毒（MCMV）史密斯株的EcoRI和HindIII片段克隆到质粒载体pACYC184中。这些克隆片段用于构建关于EcoRI和HindIII位点的基因组限制性内切酶图谱。该图谱是根据EcoRI和HindIII克隆片段的交叉杂交、克隆片段与EcoRI和HindIII的双酶切以及克隆的HindIII片段与用EcoRI切割的MCMV DNA Southern印迹杂交的数据构建的。根据我们的图谱数据，我们确定MCMV基因组的长度约为240 kbp。该基因组似乎不会发生倒位，并且缺乏可检测到的重复序列。获得了一个包含两个HindIII末端的HindIII克隆片段。该片段的存在可能与MCMV基因组的复制模式有关。
