Gharakhanian E, Takahashi J, Kasamatsu H
Virology. 1987 Apr;157(2):440-8. doi: 10.1016/0042-6822(87)90286-8.
To identify the moiety responsible for nuclear localization of the SV40 structural protein Vp3 in its natural environment, a transfection vector containing the entire coding regions of Vp2, Vp3, and agnoprotein, and one-third of the coding region of Vp1, was constructed. Several mutations were introduced into the plasmid and the subcellular distribution of Vp3 or mutant Vp3 was examined following DEAE-dextran-mediated DNA transfection into TC7 cells. Our study shows that Vp3 is synthesized and is transported into the nucleus in the absence of Vp2, agnoprotein, and intact Vp1. However, in the absence of its carboxyl-terminal 35 amino acids, the truncated Vp3 is limited to a cytoplasmic and perinuclear accumulation. Thus, the carboxyl 35 amino acids of Vp3 are required for its nuclear localization and may contain a nuclear accumulation signal.
为了在其自然环境中鉴定负责SV40结构蛋白Vp3核定位的部分，构建了一个转染载体，该载体包含Vp2、Vp3和agnoprotein的完整编码区以及Vp1编码区的三分之一。将几个突变引入质粒，并在通过DEAE-葡聚糖介导的DNA转染进入TC7细胞后，检查Vp3或突变型Vp3的亚细胞分布。我们的研究表明，在没有Vp2、agnoprotein和完整Vp1的情况下，Vp3被合成并转运到细胞核中。然而，在没有其羧基末端35个氨基酸的情况下，截短的Vp3局限于细胞质和核周积累。因此，Vp3的羧基末端35个氨基酸是其核定位所必需的，并且可能包含一个核积累信号。
