花椰菜花叶病毒外壳蛋白的核靶向
Nuclear targeting of the cauliflower mosaic virus coat protein.
作者信息
Leclerc D, Chapdelaine Y, Hohn T
机构信息
Friedrich Miescher Institut, CH-4002 Basel, Switzerland.
出版信息
J Virol. 1999 Jan;73(1):553-60. doi: 10.1128/JVI.73.1.553-560.1999.
Abstract
The entry of the viral genomic DNA of cauliflower mosaic virus into the nucleus is a critical step of viral infection. We have shown by transient expression in plant protoplasts that the viral coat protein (CP), which is processed from the product of open reading frame IV, contains an N-terminal nuclear localization signal (NLS). The NLS is exposed on the surface of the virion and is thus available for interaction with a putative NLS receptor. Phosphorylation of the matured CP did not influence the nuclear localization of the protein but improved protein stability. Mutation of the NLS completely abolished viral infectivity, thus indicating its importance in the virus life cycle. The NLS seems to be regulated by the N terminus of the precapsid, which inhibits its nuclear targeting. This regulation could be important in allowing virus assembly in the cytoplasm.
摘要
花椰菜花叶病毒的病毒基因组DNA进入细胞核是病毒感染的关键步骤。我们通过在植物原生质体中的瞬时表达表明，从开放阅读框IV的产物加工而来的病毒外壳蛋白（CP）含有一个N端核定位信号（NLS）。该NLS暴露在病毒粒子表面，因此可用于与假定的NLS受体相互作用。成熟CP的磷酸化不影响该蛋白的核定位，但提高了蛋白稳定性。NLS的突变完全消除了病毒的感染性，从而表明其在病毒生命周期中的重要性。NLS似乎受前衣壳N端的调节，前衣壳N端抑制其核靶向。这种调节对于允许病毒在细胞质中组装可能很重要。
相似文献
1
Nuclear targeting of the cauliflower mosaic virus coat protein.花椰菜花叶病毒外壳蛋白的核靶向
J Virol. 1999 Jan;73(1):553-60. doi: 10.1128/JVI.73.1.553-560.1999.
2
The product of ORF III in cauliflower mosaic virus interacts with the viral coat protein through its C-terminal proline rich domain.花椰菜花叶病毒中开放阅读框III的产物通过其富含脯氨酸的C末端结构域与病毒外壳蛋白相互作用。
Virus Genes. 2001 Mar;22(2):159-65. doi: 10.1023/a:1008121228637.
3
Regulated nuclear targeting of cauliflower mosaic virus.
J Gen Virol. 2002 Jul;83(Pt 7):1783-1790. doi: 10.1099/0022-1317-83-7-1783.
4
Mutation of capsid protein phosphorylation sites abolishes cauliflower mosaic virus infectivity.衣壳蛋白磷酸化位点的突变会消除花椰菜花叶病毒的感染性。
J Virol. 2002 Nov;76(22):11748-52. doi: 10.1128/jvi.76.22.11748-11752.2002.
5
Phosphorylation of the termini of Cauliflower mosaic virus precapsid protein is important for productive infection.花椰菜花叶病毒前衣壳蛋白末端的磷酸化对于有效感染至关重要。
Mol Plant Microbe Interact. 2007 Jun;20(6):648-58. doi: 10.1094/MPMI-20-6-0648.
6
Interaction between the open reading frame III product and the coat protein is required for transmission of cauliflower mosaic virus by aphids.花椰菜花叶病毒通过蚜虫传播需要开放阅读框III产物与外壳蛋白之间的相互作用。
J Virol. 2001 Jan;75(1):100-6. doi: 10.1128/JVI.75.1.100-106.2001.
7
Requirement of cauliflower mosaic virus open reading frame VI product for viral gene expression and multiplication in turnip protoplasts.花椰菜花叶病毒开放阅读框VI产物在芜菁原生质体中对病毒基因表达和增殖的需求。
Microbiol Immunol. 1998;42(5):377-86. doi: 10.1111/j.1348-0421.1998.tb02298.x.
8
Aphid transmission of cauliflower mosaic virus requires the viral PIII protein.花椰菜花叶病毒通过蚜虫传播需要病毒的PIII蛋白。
EMBO J. 1999 Dec 15;18(24):7077-85. doi: 10.1093/emboj/18.24.7077.
9
The open reading frame VI product of Cauliflower mosaic virus is a nucleocytoplasmic protein: its N terminus mediates its nuclear export and formation of electron-dense viroplasms.花椰菜花叶病毒的开放阅读框VI产物是一种核质蛋白：其N端介导其核输出和电子致密病毒质的形成。
Plant Cell. 2005 Mar;17(3):927-43. doi: 10.1105/tpc.104.029017.
10
Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.花椰菜花叶病毒外壳蛋白与前基因组RNA前导序列的相互作用。
J Virol. 2000 Mar;74(5):2067-72. doi: 10.1128/jvi.74.5.2067-2072.2000.
引用本文的文献
1
Setting Up Shop: The Formation and Function of the Viral Factories of .开设工厂：……病毒工厂的形成与功能
Front Plant Sci. 2017 Oct 30;8:1832. doi: 10.3389/fpls.2017.01832. eCollection 2017.
2
RNA based viral silencing suppression in plant pararetroviruses.植物副逆转录病毒中基于RNA的病毒沉默抑制
Front Plant Sci. 2015 Jun 10;6:398. doi: 10.3389/fpls.2015.00398. eCollection 2015.
3
Top 10 plant viruses in molecular plant pathology.十大植物病毒在分子植物病理学中。
Mol Plant Pathol. 2011 Dec;12(9):938-54. doi: 10.1111/j.1364-3703.2011.00752.x. Epub 2011 Oct 21.
4
Analyses of phosphorylation events in the rubella virus capsid protein: role in early replication events.风疹病毒衣壳蛋白磷酸化事件的分析：在早期复制事件中的作用
J Virol. 2006 Jul;80(14):6917-25. doi: 10.1128/JVI.01152-05.
5
Phosphorylation of the potyvirus capsid protein by protein kinase CK2 and its relevance for virus infection.马铃薯Y病毒衣壳蛋白的蛋白激酶CK2磷酸化作用及其与病毒感染的相关性。
Plant Cell. 2003 Sep;15(9):2124-39. doi: 10.1105/tpc.012567.
6
Mutation of capsid protein phosphorylation sites abolishes cauliflower mosaic virus infectivity.衣壳蛋白磷酸化位点的突变会消除花椰菜花叶病毒的感染性。
J Virol. 2002 Nov;76(22):11748-52. doi: 10.1128/jvi.76.22.11748-11752.2002.
7
Plant DNA viruses and gene silencing.植物DNA病毒与基因沉默。
Plant Mol Biol. 2000 Jun;43(2-3):307-22. doi: 10.1023/a:1006408101473.
8
Interaction of the cauliflower mosaic virus coat protein with the pregenomic RNA leader.花椰菜花叶病毒外壳蛋白与前基因组RNA前导序列的相互作用。
J Virol. 2000 Mar;74(5):2067-72. doi: 10.1128/jvi.74.5.2067-2072.2000.
本文引用的文献
1
Cauliflower mosaic virus in the nucleus of Nicotiana.烟草细胞核中的花椰菜花叶病毒。
Virology. 1985 Oct 15;146(1):141-5. doi: 10.1016/0042-6822(85)90061-3.
2
Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis.植物膜蛋白的二维凝胶电泳分析中的增溶。
Plant Physiol. 1986 Jul;81(3):802-6. doi: 10.1104/pp.81.3.802.
3
Cauliflower mosaic virus coat protein is phosphorylated in vitro by a virion-associated protein kinase.花椰菜花叶病毒外壳蛋白在病毒粒子相关蛋白激酶的作用下可发生体外磷酸化。
Proc Natl Acad Sci U S A. 1987 Apr;84(7):1824-8. doi: 10.1073/pnas.84.7.1824.
4
In vitro expression of cauliflower mosaic virus genes.在体外用菜花 mosaic 病毒基因表达。
EMBO J. 1988 Feb;7(2):309-17. doi: 10.1002/j.1460-2075.1988.tb02814.x.
5
Viral protein R regulates nuclear import of the HIV-1 pre-integration complex.病毒蛋白R调节HIV-1整合前复合物的核输入。
EMBO J. 1998 Feb 16;17(4):909-17. doi: 10.1093/emboj/17.4.909.
6
Nuclear import of DNA in digitonin-permeabilized cells.
J Cell Sci. 1997 Sep;110 ( Pt 18):2323-31. doi: 10.1242/jcs.110.18.2323.
7
HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import.HIV-1对非分裂细胞的感染：病毒基质蛋白氨基末端碱性区域对Gag加工很重要，但对进入后核输入不重要的证据。
EMBO J. 1997 Aug 1;16(15):4531-9. doi: 10.1093/emboj/16.15.4531.
8
Phosphorylation of residue 131 of HIV-1 matrix is not required for macrophage infection.HIV-1基质蛋白第131位残基的磷酸化对于巨噬细胞感染并非必需。
Cell. 1997 Jan 24;88(2):171-3; discussion 173-4. doi: 10.1016/s0092-8674(00)81836-x.
9
In vitro model for the nuclear transport of the hepadnavirus genome.乙型肝炎病毒基因组核转运的体外模型
J Virol. 1997 Feb;71(2):1310-6. doi: 10.1128/JVI.71.2.1310-1316.1997.
10
Effect of M1 protein and low pH on nuclear transport of influenza virus ribonucleoproteins.M1蛋白和低pH值对流感病毒核糖核蛋白核转运的影响。
J Virol. 1996 Dec;70(12):8391-401. doi: 10.1128/JVI.70.12.8391-8401.1996.
Zotero 插件