从冠状病毒小鼠肝炎病毒A59株的一个非结构基因体外合成两种多肽。
In vitro synthesis of two polypeptides from a nonstructural gene of coronavirus mouse hepatitis virus strain A59.
作者信息
Budzilowicz C J, Weiss S R
出版信息
Virology. 1987 Apr;157(2):509-15. doi: 10.1016/0042-6822(87)90293-5.
Abstract
The complete nucleotide sequence of nonstructural gene 5 of coronavirus mouse hepatitis virus (MHV) strain A59 has been determined. This sequence contains two potential open reading frames which overlap by five nucleotides. The putative protein products predicted from the sequence are a basic 13,000-Da polypeptide and a 9600-Da polypeptide containing an unusually long hydrophobic amino terminus. RNAs transcribed in vitro from DNAs containing each of the open reading frames in pGEM vectors direct the synthesis in vitro of polypeptides of the sizes predicted by the sequence. An RNA transcript containing both of the open reading frames directs the synthesis primarily of the polypeptide corresponding to the downstream open reading frame. These data suggest that MHV-A59 messenger RNA 5 potentially encodes two proteins and may be preferentially translated from an internal AUG initiation codon.
摘要
已确定冠状病毒小鼠肝炎病毒（MHV）A59株非结构基因5的完整核苷酸序列。该序列包含两个潜在的开放阅读框，它们重叠五个核苷酸。从该序列预测的假定蛋白质产物是一种碱性的13000道尔顿多肽和一种9600道尔顿多肽，后者含有异常长的疏水氨基末端。从含有pGEM载体中每个开放阅读框的DNA体外转录的RNA指导体外合成序列预测大小的多肽。包含两个开放阅读框的RNA转录本主要指导对应于下游开放阅读框的多肽的合成。这些数据表明，MHV-A59信使RNA 5可能编码两种蛋白质，并且可能优先从内部AUG起始密码子进行翻译。
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