Department of Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Department of Biology, University of Southern Denmark, 5230 Odense M, Denmark.
Mar Pollut Bull. 2018 Oct;135:1090-1100. doi: 10.1016/j.marpolbul.2018.08.043. Epub 2018 Aug 28.
This study investigates the extra UV fluence needed to meet the International Maritime Organisation's ballast water discharge standards for the 10-50 μm size-class using the approved vital stain (VS) method compared to the Most Probable Number (MPN) method for organism viability assessment. Low- and medium pressure UV collimated beam treatments were applied to natural algae collected in temperate and tropical water environments and analysed using both methods. About 10 times higher UV fluence was required to meet discharge standards when using VS compared to MPN. Implementing a dark-hold period after UV treatments decreased algal viability. Length of dark-hold period to meet discharge standards decreased with increasing UV fluence. No significant differences between temperate and tropical samples were observed. The results showed that UV treated algae assessed using the VS method could meet discharge standards by increasing fluence and/or introducing a dark-hold period.
本研究调查了使用经批准的活体染色（VS）方法与最可能数（MPN）方法相比，为满足 10-50μm 粒径国际海事组织压载水排放标准而需要额外的紫外线辐照剂量，以评估生物的存活能力。对在温带和热带水域采集的天然藻类进行了低压和中压紫外线平行光束处理，并分别使用这两种方法进行了分析。与 MPN 相比，VS 法满足排放标准所需的紫外线辐照剂量要高出约 10 倍。紫外线处理后进行暗保持期可降低藻类的活力。暗保持期的长度随着紫外线辐照剂量的增加而减少，以满足排放标准。在温带和热带样本之间没有观察到显著差异。结果表明，通过增加辐照剂量和/或引入暗保持期，使用 VS 方法评估的紫外线处理藻类可以达到排放标准。
