Department of Biology, University of Southern Denmark, Odense M, Denmark.
Department of Biology, University of Southern Denmark, Odense M, Denmark.
Mar Pollut Bull. 2019 Jul;144:253-264. doi: 10.1016/j.marpolbul.2019.04.072. Epub 2019 May 16.
To challenge ballast water treatment system (BWTS) efficacy for organisms in the size-class 10-50 μm, intake concentration during tests must reach certain minimum requirements. Often, natural concentrations are too low to meet intake requirements and standard test organisms (STOs) are added. We tested the robustness of Tetraselmis suecica and Odontella sp. to a range of UV-treatments to explore fluences needed to meet the IMO discharge standard (<10 org. ml) evaluated using two viability assessment methods. To meet discharge standards, fluences of >1000 mJ cm were required using vital stain whereas 135-500 mJ cm were needed using regrowth assays. Besides, results suggest that T. suecica and Odontella sp. were at least as robust as natural algae towards UV-treatments. We suggest the advantageous use of these species as STOs in test water to support intake water requirements and to obtain more conservative validation of UV-based BWTS to ensure more environmental protective procedures.
为了挑战 10-50μm 大小类别的压载水处理系统（BWTS）对生物的效果，测试期间的进水浓度必须达到一定的最低要求。通常，自然浓度太低，无法满足进水要求，因此需要添加标准测试生物（STO）。我们测试了马尾藻和星杆藻对一系列紫外线处理的稳健性，以探索满足 IMO 排放标准（<10 个生物/ml）所需的紫外线剂量，使用两种生存力评估方法进行评估。使用活细胞染色剂，需要超过 1000mJ/cm 的剂量才能达到排放标准，而使用再生测定法则需要 135-500mJ/cm 的剂量。此外，结果表明，马尾藻和星杆藻对紫外线处理的抵抗力至少与天然藻类一样强。我们建议将这些物种作为 STO 用于测试水，以支持进水要求，并更保守地验证基于紫外线的 BWTS，以确保更环保的处理程序。
